« Previous AbstractIdentification of the differences in olfactory system between male and female oriental tobacco budworm Helicoverpa assulta    Next AbstractEffects of temporary rearing with organic selenium on the muscle flavor and texture properties of largemouth bass (Micropterus salmonides) »

BMC Genomics

Title:		Antennal transcriptome analysis of olfactory genes and tissue expression profiling of odorant binding proteins in Semanotus bifasciatus (cerambycidae: coleoptera)
Author(s):		Li H; Hao E; Li Y; Yang H; Sun P; Lu P; Qiao H;
Address:		"The Key Laboratory for Silviculture and Conservation of the Ministry of Education, School of Forestry, Beijing Forestry University, 35 Qinghua Dong Road, Haidian District, Beijing, 100083, People's Republic of China. The Key Laboratory for Silviculture and Conservation of the Ministry of Education, School of Forestry, Beijing Forestry University, 35 Qinghua Dong Road, Haidian District, Beijing, 100083, People's Republic of China. lpengfei224@126.com. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing, 100193, People's Republic of China. qhl193314@163.com"
Journal Title:		BMC Genomics
Year:		2022
Volume:		20220622
Issue:		1
Page Number:		461 -
DOI:		10.1186/s12864-022-08655-w
ISSN/ISBN:		1471-2164 (Electronic) 1471-2164 (Linking)
Abstract:		"BACKGROUND: Insect olfactory proteins can transmit chemical signals in the environment that serve as the basis for foraging, mate searching, predator avoidance and oviposition selection. Semanotus bifasciatus is an important destructive borer pest, but its olfactory mechanism is not clear. We identified the chemosensory genes of S. bifasciatus in China, then we conducted a phylogenetic analysis of the olfactory genes of S. bifasciatus and other species. And the expression profiles of odorant binding proteins (OBPs) genes in different tissues and different genders of S. bifasciatus were determined by quantitative real-time PCR for the first time. RESULTS: A total of 32 OBPs, 8 chemosensory proteins (CSPs), 71 odorant receptors (ORs), 34 gustatory receptors (GRs), 18 ionotropic receptors (IRs), and 3 sensory neuron membrane proteins (SNMPs) were identified. In the tissue expression analysis of OBP genes, 7 OBPs were higher expressed in antennae, among them, SbifOBP2, SbifOBP3, SbifOBP6, SbifOBP7 and SbifOBP20 were female-biased expression, while SbifOBP1 was male-biased expression and SbifOBP22 was no-biased expression in antennae. In addition, the expressed levels of SbifOBP4, SbifOBP12, SbifOBP15, SbifOBP27 and SbifOBP29 were very poor in the antennae, and SbifOBP4 and SbifOBP29 was abundant in the head or legs, and both of them were male-biased expression. While SbifOBP15 was highly expressed only at the end of the abdomen with its expression level in females three times than males. Other OBPs were expressed not only in antennae but also in various tissues. CONCLUSION: We identified 166 olfactory genes from S. bifasciatus, and classified these genes into groups and predicted their functions by phylogenetic analysis. The majority of OBPs were antenna-biased expressed, which are involved in odor recognition, sex pheromone detection, and/or host plant volatile detection. However, also some OBPs were detected biased expression in the head, legs or end of the abdomen, indicating that they may function in the different physiological processes in S. bifasciatus"
Keywords:		"Animals Arthropod Antennae/metabolism *Coleoptera/genetics/metabolism Female Gene Expression Profiling Insect Proteins/genetics/metabolism Male Odorants Phylogeny *Receptors, Odorant/genetics/metabolism Transcriptome Antennal transcriptome Odorant binding;"
Notes:		"MedlineLi, Han Hao, Enhua Li, Yini Yang, Huan Sun, Piao Lu, Pengfei Qiao, Haili eng Grant No. 31570643, 81774015/National Natural Science Foundation of China/ 2017YFD0600103/National Key Research and Development Program of China/ England 2022/06/23 BMC Genomics. 2022 Jun 22; 23(1):461. doi: 10.1186/s12864-022-08655-w"