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Comp Biochem Physiol Part D Genomics Proteomics

Title:		Identification and expression profiling of chemosensory membrane protein genes in Achelura yunnanensis (Lepidoptera: Zygaenidae)
Author(s):		Li GC; Nuo SM; Wang ZQ; Yang AJ; Liu NY;
Address:		"Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China. Electronic address: naiyong_2013@163.com"
Journal Title:		Comp Biochem Physiol Part D Genomics Proteomics
Year:		2021
Volume:		20210706
Issue:
Page Number:		100876 -
DOI:		10.1016/j.cbd.2021.100876
ISSN/ISBN:		1878-0407 (Electronic) 1744-117X (Linking)
Abstract:		"During the past decade, antennal transcriptome sequencing has been applied to at least 50 species from 16 families of the Lepidoptera order of insects, emphasizing the identification and characterization of chemosensory-related genes. However, little is known about the chemosensory genes in the Zygaenidae family of Lepidoptera. Herein, we report the transmembrane protein gene repertoires involved in chemoreception from Achelura yunnanensis (Lepidoptera: Zygaenidae) through transcriptome sequencing, bioinformatics, phylogenetics and polymerase chain reaction (PCR) approaches. Transcriptome analysis led to the generation of 555.47 million clean reads and accumulation of 83.30 gigabases of data. From this transcriptome, 132 transcripts encoding 69 odorant receptors (ORs), 33 gustatory receptors (GRs), 26 ionotropic receptors (IRs), and four sensory neuron membrane proteins (SNMPs) were identified, 69 of which were full-length sequences. Notably, the number of SNMPs in A. yunnanensis was the largest set in Lepidoptera to date. Phylogenetic analysis combined with sequence homology highlighted several conserved groups of chemoreceptors, including pheromone receptors (a so-called pheromone receptor (PR) clade: AyunOR50 and novel PR members: AyunOR39 and OR40), a phenylacetaldehyde-sensing OR (AyunOR28), carbon dioxide receptors (AyunGR1-3), and antennal IRs (13 A-IRs). In addition, a Zygaenidae-specific OR expansion was observed, including 15 A. yunnanensis members. Expression profiles revealed 99 detectable chemosensory genes in the antennae and 20 in the reproductive tissues, some of which displayed a sex-biased expression. This study identifies potential olfactory molecular candidates for sensing sex pheromones, phenylacetaldehyde or other odorants, and provides preliminary evidence for the putative reproductive function of chemosensory membrane protein genes in A. yunnanensis"
Keywords:		"Animals Arthropod Antennae/metabolism Gene Expression Profiling Insect Proteins/genetics/metabolism *Lepidoptera/genetics/metabolism Phylogeny *Receptors, Odorant/genetics Transcriptome Achelura yunnanensis Chemosensory receptor Olfaction Reproduction Sen;"
Notes:		"MedlineLi, Gen-Ceng Nuo, Shu-Mei Wang, Zheng-Quan Yang, An-Jin Liu, Nai-Yong eng Research Support, Non-U.S. Gov't Netherlands 2021/07/12 Comp Biochem Physiol Part D Genomics Proteomics. 2021 Dec; 40:100876. doi: 10.1016/j.cbd.2021.100876. Epub 2021 Jul 6"