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Plant Cell Environ

Title:		Expression of lima bean terpene synthases in rice enhances recruitment of a beneficial enemy of a major rice pest
Author(s):		Li F; Li W; Lin YJ; Pickett JA; Birkett MA; Wu K; Wang G; Zhou JJ;
Address:		"Institute of Plant Protection, Chinese Academy of Agricultural Science, No. 2 West Yuanmingyuan Road, Haidian District, Beijing, 100193, China. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. Huazhong Agricultural University, No. 1 Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, China. Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK"
Journal Title:		Plant Cell Environ
Year:		2018
Volume:		20170714
Issue:		1
Page Number:		111 - 120
DOI:		10.1111/pce.12959
ISSN/ISBN:		1365-3040 (Electronic) 0140-7791 (Linking)
Abstract:		"Volatile terpenoids play a key role in plant defence against herbivory by attracting parasitic wasps. We identified seven terpene synthase genes from lima bean, Phaseolus lunatus L. following treatment with either the elicitor alamethicin or spider mites, Tetranychus cinnabarinus. Four of the genes (Pltps2, Pltps3, Pltps4 and Pltps5) were up-regulated with their derived proteins phylogenetically clustered in the TPS-g subfamily and PlTPS3 positioned at the base of this cluster. Recombinant PlTPS3 was able to convert geranyl diphosphate and farnesyl diphosphate to linalool and (E)-nerolidol, the latter being precursor of the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). Recombinant PlTPS4 showed a different substrate specificity and produced linalool and (E)-nerolidol, as well as (E,E)-geranyllinalool from geranylgeranyl diphosphate. Transgenic rice expressing Pltps3 emitted significantly more (S)-linalool and DMNT than wild-type plants, whereas transgenic rice expressing Pltps4 produced (S)-linalool, DMNT and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT). In laboratory bioassays, female Cotesia chilonis, the natural enemy of the striped rice stemborer, Chilo suppressalis, were significantly attracted to the transgenic plants and their volatiles. We further confirmed this with synthetic blends mimicking natural rice volatile composition. Our study demonstrates that the transformation of rice to produce volatile terpenoids has the potential to enhance plant indirect defence through natural enemy recruitment"
Keywords:		"Alkyl and Aryl Transferases/genetics/*metabolism Animals Behavior, Animal Fabaceae/*enzymology Female Gas Chromatography-Mass Spectrometry *Gene Expression Regulation, Plant Genes, Plant Oryza/*genetics/*parasitology Parasites/physiology Phylogeny Plant P;"
Notes:		"MedlineLi, Fengqi Li, Wei Lin, Yong-Jun Pickett, John A Birkett, Michael A Wu, Kongming Wang, Guirong Zhou, Jing-Jiang eng BB/E015794/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom BB/G004781/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom 2017/04/04 Plant Cell Environ. 2018 Jan; 41(1):111-120. doi: 10.1111/pce.12959. Epub 2017 Jul 14"