| Title: | Noninvasive Pregestational Genetic Testing of Embryos Using Smart Sensors Array |
| Author(s): | Abu Raya YS; Srebnik N; Rubinstein E; Schonberger O; Broza YY; Suschinel R; Haick H; Ionescu R; |
| Address: | "Department of Chemical Engineering and Russel Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, 3200003 Haifa, Israel. In Vitro Fertilization Unit, Shaare Zedek Medical Center, Hebrew University Medical School, 9112102 Jerusalem, Israel. Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 51006 Tartu, Estonia" |
| DOI: | 10.1021/acssensors.2c01492 |
| ISSN/ISBN: | 2379-3694 (Electronic) 2379-3694 (Linking) |
| Abstract: | "Pregestational genetic testing of embryos is the conventional tool in detecting genetic disorders (fetal aneuploidy and monogenic disorders) for in vitro fertilization (IVF) procedures. The accepted clinical practice for genetic testing still depends on biopsy, which has the potential to harm the embryo. Noninvasive genetic prenatal testing has not yet been achieved. In this study, embryos with common genetic disorders created through IVF were tested with an artificially intelligent nanosensor array. Volatile organic compounds emitted by the culture fluid of embryos were analyzed with chemical gas sensors. The obtained results showed significant discrimination between the embryos with different genetic diseases and their wild-types. Embryos were obtained from the same clinical center for avoiding differences based on clinical and demographical characteristics. The achieved discrimination accuracy was 81% for PKD disease, 90% for FRAX disease, 85% for HOCM disease, 90% for BRCA disease, and 100% for HSCR disease. These proof-of-concept findings might launch the development of a noninvasive approach for early assessment of embryos by examining the culture fluid of the embryos, potentially enabling noninvasive diagnosis and screening of genetic diseases for IVF" |
| Keywords: | Pregnancy Female Humans *Preimplantation Diagnosis/methods Blastocyst Genetic Testing Aneuploidy Fertilization in Vitro/methods electronic nose embryos genetic disorders in vitro fertilization volatile organic compounds; |
| Notes: | "MedlineAbu Raya, Yasmin Shibli Srebnik, Naama Rubinstein, Esther Schonberger, Oshrat Broza, Yoav Y Suschinel, Raluca Haick, Hossam Ionescu, Radu eng Letter Research Support, Non-U.S. Gov't 2022/11/15 ACS Sens. 2022 Nov 25; 7(11):3265-3271. doi: 10.1021/acssensors.2c01492. Epub 2022 Nov 14" |
