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J Breath Res

Title:		Headspace analysis of mesothelioma cell lines differentiates biphasic and epithelioid sub-types
Author(s):		Little LD; Carolan VA; Allen KE; Cole LM; Haywood-Small SL;
Address:		"Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, United Kingdom"
Journal Title:		J Breath Res
Year:		2020
Volume:		20200926
Issue:		4
Page Number:		46011 -
DOI:		10.1088/1752-7163/abaaff
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Linking)
Abstract:		"Malignant mesothelioma (MM) is an incurable cancer. MM is often misdiagnosed, with a poor 5-year survival and limited treatment options. The discovery of endogenous volatile organic compounds (VOCs) is required in order to accelerate the development of a breath test as an alternative to conventional MM diagnosis. For the first time, this study used solid-phase microextraction and gas chromatography-mass spectrometry to identify VOCs released directly from the biphasic MM cell line MSTO-211H and the epithelioid MM cell line NCI-H28 as well as the non-malignant mesothelial cell line MET-5A. Multivariate statistical analysis showed separation between MSTO-211H, NCI-H28 and MET-5A results. 2-ethyl-1-hexanol was significantly increased in both MSTO-211H and NCI-H28 cells compared to MET-5A controls. In addition, ethyl propionate and cyclohexanol were significantly increased in MSTO-211H cells and dodecane was significantly increased in NCI-H28 cells. This is the first study reporting headspace analysis of these MM cell lines and the first to consider the effects of mesothelioma sub-type on VOC profile. Current results further highlight the potential for a diagnostic mesothelioma breath test as well as providing proof of concept for the differentiation between biphasic and epithelioid mesothelioma based on VOC profiles"
Keywords:		"Breath Tests Cell Count Cell Line, Tumor Cell Survival *Gas Chromatography-Mass Spectrometry Humans Least-Squares Analysis Mesothelioma/*diagnosis/*pathology Principal Component Analysis Volatile Organic Compounds/analysis;"
Notes:		"MedlineLittle, Liam David Carolan, Vikki Amanda Allen, Kathryn Elizabeth Cole, Laura Margaret Haywood-Small, Sarah Louise eng Research Support, Non-U.S. Gov't England 2020/07/31 J Breath Res. 2020 Sep 26; 14(4):046011. doi: 10.1088/1752-7163/abaaff"