| Title: | The ERD2 gene determines the specificity of the luminal ER protein retention system |
| Author(s): | Lewis MJ; Sweet DJ; Pelham HR; |
| Address: | "MRC Laboratory of Molecular Biology, Cambridge, England" |
| DOI: | 10.1016/0092-8674(90)90699-f |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "Luminal ER proteins carry a signal at their C terminus that prevents their secretion; in S. cerevisiae this signal is the tetrapeptide HDEL. Indirect evidence suggests that HDEL is recognized by a receptor that retrieves ER proteins from the secretory pathway and returns them to the ER, and a candidate for this receptor is the product of the ERD2 gene (see accompanying paper). We show here that presumptive ER proteins from the budding yeast K. lactis can terminate either with HDEL or, in the case of BiP, with DDEL. S. cerevisiae does not efficiently recognize DDEL as a retention signal, but exchange of its ERD2 gene for the corresponding gene from K. lactis allows equal recognition of DDEL and HDEL. Thus the specificity of the retention system is determined by the ERD2 gene. We conclude that ERD2 encodes the receptor that sorts luminal ER proteins" |
| Keywords: | "Amino Acid Sequence Base Sequence Cloning, Molecular Endoplasmic Reticulum/*metabolism Fungal Proteins/*genetics *Genes, Fungal Kluyveromyces/*genetics Mating Factor Membrane Proteins/*genetics Molecular Sequence Data Peptides/genetics Protein Precursors/;" |
| Notes: | "MedlineLewis, M J Sweet, D J Pelham, H R eng Comparative Study 1990/06/29 Cell. 1990 Jun 29; 61(7):1359-63. doi: 10.1016/0092-8674(90)90699-f" |
