| Title: | Identifying volatile metabolite signatures for the diagnosis of bacterial respiratory tract infection using electronic nose technology: A pilot study |
| Author(s): | Lewis JM; Savage RS; Beeching NJ; Beadsworth MBJ; Feasey N; Covington JA; |
| Address: | "Tropical and Infectious Disease Unit, Royal Liverpool University Hospital, Liverpool, United Kingdom. Wellcome Trust Liverpool Glasgow Centre for Global Health Research, Liverpool, United Kingdom. Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom. Department of Statistics, University of Warwick, Coventry, United Kingdom. School of Engineering, University of Warwick, Coventry, United Kingdom" |
| DOI: | 10.1371/journal.pone.0188879 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "OBJECTIVES: New point of care diagnostics are urgently needed to reduce the over-prescription of antimicrobials for bacterial respiratory tract infection (RTI). We performed a pilot cross sectional study to assess the feasibility of gas-capillary column ion mobility spectrometer (GC-IMS), for the analysis of volatile organic compounds (VOC) in exhaled breath to diagnose bacterial RTI in hospital inpatients. METHODS: 71 patients were prospectively recruited from the Acute Medical Unit of the Royal Liverpool University Hospital between March and May 2016 and classified as confirmed or probable bacterial or viral RTI on the basis of microbiologic, biochemical and radiologic testing. Breath samples were collected at the patient's bedside directly into the electronic nose device, which recorded a VOC spectrum for each sample. Sparse principal component analysis and sparse logistic regression were used to develop a diagnostic model to classify VOC spectra as being caused by bacterial or non-bacterial RTI. RESULTS: Summary area under the receiver operator characteristic curve was 0.73 (95% CI 0.61-0.86), summary sensitivity and specificity were 62% (95% CI 41-80%) and 80% (95% CI 64-91%) respectively (p = 0.00147). CONCLUSIONS: GC-IMS analysis of exhaled VOC for the diagnosis of bacterial RTI shows promise in this pilot study and further trials are warranted to assess this technique" |
| Keywords: | Aged Bacterial Infections/*diagnosis/microbiology *Electronic Nose Female Humans Male *Metabolomics Middle Aged Pilot Projects ROC Curve Respiratory Tract Infections/*diagnosis/microbiology Volatile Organic Compounds/*analysis; |
| Notes: | "MedlineLewis, Joseph M Savage, Richard S Beeching, Nicholas J Beadsworth, Mike B J Feasey, Nicholas Covington, James A eng WT_/Wellcome Trust/United Kingdom 204699/WT_/Wellcome Trust/United Kingdom 2017/12/19 PLoS One. 2017 Dec 18; 12(12):e0188879. doi: 10.1371/journal.pone.0188879. eCollection 2017" |
