| Title: | Discovery and Functional Study of a Novel Genomic Locus Homologous to Balpha-Mating-Type Sublocus of Lentinula edodes |
| Author(s): | Lee YJ; Kim E; Eom H; Yang SH; Choi YJ; Ro HS; |
| Address: | "Department of Bio & Medical Big Data (BK4 program) and Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea" |
| DOI: | 10.1080/12298093.2021.2001906 |
| ISSN/ISBN: | 1229-8093 (Print) 2092-9323 (Electronic) 1229-8093 (Linking) |
| Abstract: | "The interaction of mating pheromone and pheromone receptor from the B mating-type locus is the first step in the activation of the mushroom mating signal transduction pathway. The B mating-type locus of Lentinula edodes is composed of Balpha and Bbeta subloci, each of which contains genes for mating pheromone and pheromone receptor. Allelic variations in both subloci generate multiple B mating-types through which L. edodes maintains genetic diversity. In addition to the B mating-type locus, our genomic sequence analysis revealed the presence of a novel chromosomal locus 43.3 kb away from the B mating-type locus, containing genes for a pair of mating pheromones (PHBN1 and PHBN2) and a pheromone receptor (RCBN). The new locus (Balpha-N) was homologous to the Balpha sublocus, but unlike the multiallelic Balpha sublocus, it was highly conserved across the wild and cultivated strains. The interactions of RcbN with various mating pheromones from the B and Balpha-N mating-type loci were investigated using yeast model that replaced endogenous yeast mating pheromone receptor STE2 with RCBN. The yeast mating signal transduction pathway was only activated in the presence of PHBN1 or PHBN2 in the RcbN producing yeast, indicating that RcbN interacts with self-pheromones (PHBN1 and PHBN2), not with pheromones from the B mating-type locus. The biological function of the Balpha-N locus was suggested to control the expression of A mating-type genes, as evidenced by the increased expression of two A-genes HD1 and HD2 upon the treatment of synthetic PHBN1 and PHBN2 peptides to the monokaryotic strain of L. edodes" |
| Keywords: | Lentinula edodes Mating pheromone activation receptor; |
| Notes: | "PubMed-not-MEDLINELee, Yun Jin Kim, Eunbi Eom, Hyerang Yang, Seong-Hyeok Choi, Yeon Jae Ro, Hyeon-Su eng Korea (South) 2022/01/18 Mycobiology. 2021 Dec 6; 49(6):582-588. doi: 10.1080/12298093.2021.2001906. eCollection 2021" |
