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J Cell Biol

Title:		"Fission yeast myosin-I, Myo1p, stimulates actin assembly by Arp2/3 complex and shares functions with WASp"
Author(s):		Lee WL; Bezanilla M; Pollard TD;
Address:		"Graduate Program in Biochemistry, Cellular and Molecular Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA"
Journal Title:		J Cell Biol
Year:		2000
Volume:		151
Issue:		4
Page Number:		789 - 800
DOI:		10.1083/jcb.151.4.789
ISSN/ISBN:		0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking)
Abstract:		"Fission yeast myo1(+) encodes a myosin-I with all three tail homology domains (TH1, 2, 3) found in typical long-tailed myosin-Is. Myo1p tail also contains a COOH-terminal acidic region similar to the A-domain of WASp/Scar proteins and other fungal myosin-Is. Our analysis shows that Myo1p and Wsp1p, the fission yeast WASp-like protein, share functions and cooperate in controlling actin assembly. First, Myo1p localizes to cortical patches enriched at tips of growing cells and at sites of cell division. Myo1p patches partially colocalize with actin patches and are dependent on an intact actin cytoskeleton. Second, although deletion of myo1(+) is not lethal, Deltamyo1 cells have actin cytoskeletal defects, including loss of polarized cell growth, delocalized actin patches, and mating defects. Third, additional disruption of wsp1(+) is synthetically lethal, suggesting that these genes may share functions. In mapping the domains of Myo1p tail that share function with Wsp1p, we discovered that a Myo1p construct with just the head and TH1 domains is sufficient for cortical localization and to rescue all Deltamyo1 defects. However, it fails to rescue the Deltamyo1 Deltawsp1 lethality. Additional tail domains, TH2 and TH3, are required to complement the double mutant. Fourth, we show that a recombinant Myo1p tail binds to Arp2/3 complex and activates its actin nucleation activity"
Keywords:		"Actin-Related Protein 2 Actin-Related Protein 3 Actins/*metabolism Amino Acid Sequence Animals *Calmodulin-Binding Proteins Cell Division Cloning, Molecular *Cytoskeletal Proteins Fungal Proteins/chemistry/genetics/*metabolism Genetic Complementation Test;"
Notes:		"MedlineLee, W L Bezanilla, M Pollard, T D eng R01 GM026132/GM/NIGMS NIH HHS/ R37 GM026132/GM/NIGMS NIH HHS/ GM-26132/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 2000/11/15 J Cell Biol. 2000 Nov 13; 151(4):789-800. doi: 10.1083/jcb.151.4.789"