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Aquat Toxicol

Title:		Application of a programmed semi-automated Ulva pertusa bioassay for testing single toxicants and stream water quality
Author(s):		Lee H; Park J; Shin K; Depuydt S; Choi S; De Saeger J; Han T;
Address:		"Department of Marine Science, Incheon National University, 119, Academy-ro, Yeonsu-gu, Incheon, 22012, Republic of Korea; Ghent University Global Campus, 119-5, Songdomunhwa-ro, Yeonsu-gu, Incheon, 21985, Republic of Korea. Ghent University Global Campus, 119-5, Songdomunhwa-ro, Yeonsu-gu, Incheon, 21985, Republic of Korea. Electronic address: Jihae.Park@ghent.ac.kr. Water Environmental Engineering Research Division, National Institute of Environmental Research, 42, Hwangyeong-ro, Seo-gu, Incheon, 22689, Republic of Korea. Ghent University Global Campus, 119-5, Songdomunhwa-ro, Yeonsu-gu, Incheon, 21985, Republic of Korea. Department of Marine Science, Incheon National University, 119, Academy-ro, Yeonsu-gu, Incheon, 22012, Republic of Korea. Department of Marine Science, Incheon National University, 119, Academy-ro, Yeonsu-gu, Incheon, 22012, Republic of Korea; Ghent University Global Campus, 119-5, Songdomunhwa-ro, Yeonsu-gu, Incheon, 21985, Republic of Korea. Electronic address: hanalgae@hanmail.net"
Journal Title:		Aquat Toxicol
Year:		2020
Volume:		20200131
Issue:
Page Number:		105426 -
DOI:		10.1016/j.aquatox.2020.105426
ISSN/ISBN:		1879-1514 (Electronic) 0166-445X (Linking)
Abstract:		"A toxicity test based on inhibition of reproduction in the green macroalga Ulva pertusa involves quantifying the change in thallus color as reproduction progresses. However, interpretation of this color change is reliant on the skill level of the examiner. This study aimed to validate a new toxicity test based on inhibition of reproduction in the green macroalga U. pertusa using a vital stain and programmed semi-automated analysis (using Image J) of the change in thallus color. The toxicity rank by inverse EC(50) values was: irgarol (0.048?ª+mg?ª+L(-1)) > Ag (0.132?ª+mg?ª+L(-1)) > As (0.172?ª+mg?ª+L(-1)) > simazine (0.378?ª+mg?ª+L(-1)) > formaldehyde (0.442?ª+mg?ª+L(-1)) > DCOIT (0.783?ª+mg?ª+L(-1)) > ZnPT (3.556?ª+mg?ª+L(-1)) > medetomidine (11.600?ª+mg?ª+L(-1)) > phenol (29.316?ª+mg?ª+L(-1)) > methanol (2,736?ª+mg?ª+L(-1)) > ethanol (3,306?ª+mg?ª+L(-1)). The sensitivity of the U. pertusa test to stream waters was similar to or lower than those of the commonly-used Lemna minor and Daphnia magna bioassays. The U. pertusa bioassay is sensitive to, and suitable for, testing various toxicants including metals, volatile organic compounds, herbicide, antifouling agents and phenol and can also be applied to testing freshwater quality after salinity adjustment"
Keywords:		"Animals Biological Assay/methods Daphnia/drug effects Environmental Monitoring/*methods Rivers/*chemistry Sensitivity and Specificity Toxicity Tests Ulva/*drug effects Water Pollutants, Chemical/*toxicity *Water Quality Bioassay Evans Blue Image analysis;"
Notes:		"MedlineLee, Hojun Park, Jihae Shin, Kisik Depuydt, Stephen Choi, Soyeon De Saeger, Jonas Han, Taejun eng Netherlands 2020/02/10 Aquat Toxicol. 2020 Apr; 221:105426. doi: 10.1016/j.aquatox.2020.105426. Epub 2020 Jan 31"