| Title: | N alpha acetylation is required for normal growth and mating of Saccharomyces cerevisiae |
| Author(s): | Lee FJ; Lin LW; Smith JA; |
| Address: | "Department of Molecular Biology, Massachusetts General Hospital, Boston" |
| DOI: | 10.1128/jb.171.11.5795-5802.1989 |
| ISSN/ISBN: | 0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking) |
| Abstract: | "Acetylation is the most frequently occurring chemical modification of the alpha-NH2 group of eucaryotic proteins and is catalyzed by N alpha-acetyltransferase. The yeast enzyme is encoded by the AAA1 (amino-terminal alpha-amino acetyltransferase) gene. A null mutation (aaa1-1) created by gene replacement, while not lethal, slows cell growth and results in heterogeneous colony morphology. In comparison with wild-type cells, aaa1-1/aaa1-1 diploids cannot enter stationary phase, are sporulation defective, and are sensitive to heat shock. In addition, the aaa1-1 mutation specifically reduces mating functions of MATa cells. These results indicate that N alpha acetylation plays a crucial role in yeast cell growth and mating" |
| Keywords: | "Acetylation Acetyltransferases/*genetics *Genes, Fungal Genotype Hot Temperature Mutation N-Terminal Acetyltransferases Pheromones/biosynthesis Plasmids Restriction Mapping Saccharomyces cerevisiae/enzymology/genetics/*growth & development Species Specifi;" |
| Notes: | "MedlineLee, F J Lin, L W Smith, J A eng Research Support, Non-U.S. Gov't 1989/11/01 J Bacteriol. 1989 Nov; 171(11):5795-802. doi: 10.1128/jb.171.11.5795-5802.1989" |
