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Peptides

Title:		"Molecular characterization of pheromone biosynthesis activating neuropeptide from the diamondback moth, Plutella xylostella (L.)"
Author(s):		Lee DW; Boo KS;
Address:		"School of Agricultural Biotechnology, Seoul National University, San 56-1 Shillim-dong, Gwanak-gu, Seoul 151-742, Republic of Korea"
Journal Title:		Peptides
Year:		2005
Volume:		20050706
Issue:		12
Page Number:		2404 - 2411
DOI:		10.1016/j.peptides.2005.04.016
ISSN/ISBN:		0196-9781 (Print) 0196-9781 (Linking)
Abstract:		"Pheromone biosynthesis activating neuropeptide (PBAN) produced in the subesophageal ganglion stimulates pheromone production in the pheromone gland. A cDNA isolated from female adult heads of the diamondback moth (Plutella xylostella (L.)) encodes 193 amino acids including PBAN, designated as Plx-PBAN, and four other neuropeptides (NPs): diapause hormone (DH) homologue, alpha-NP, beta-NP and gamma-NP. All of the peptides are amidated in their C-termini and shared a conserved motif, FXPR(or K)L structure, as reported from other PBAN cDNAs. Plx-PBAN consists of 30 amino acids, the shortest PBAN so far reported. Plx-PBAN exhibited below 50% homology, compared with other known PBANs. The Plx-DH homologue is structurally different from DH of Bombyx mori. The length of Plx-beta-NP (16 amino acids) was the shortest and showed relatively low similarity, whereas gamma-NP (10 amino acids in length) was the longest among examined gamma-NPs. When female adults were injected with synthetic Plx-PBAN, pheromone production showed a maximal increase 1h post-injection. RT-PCR screening revealed that Plx-PBAN cDNA was expressed in all examined body parts, with the highest expression level in the head of female adults. Analysis of RT-PCR products indicated the Plx-PBAN sequence was identical in all examined body parts of both sexes. Phylogenetic analysis revealed that the Plx-PBAN gene is distantly related to other PBANs, demonstrated by the relatively low similarity"
Keywords:		"Amino Acid Sequence Animals Cloning, Molecular Female Insect Proteins/*genetics/metabolism Molecular Sequence Data Moths/*genetics/metabolism Neuropeptides/*genetics/metabolism Pheromones/biosynthesis Sequence Homology, Amino Acid;"
Notes:		"MedlineLee, Dae-Weon Boo, Kyung Saeng eng Research Support, Non-U.S. Gov't 2005/07/12 Peptides. 2005 Dec; 26(12):2404-11. doi: 10.1016/j.peptides.2005.04.016. Epub 2005 Jul 6"