| Title: | Development of a simultaneous multiple solid-phase microextraction-single shot-gas chromatography/mass spectrometry method and application to aroma profile analysis of commercial coffee |
| Author(s): | Lee C; Lee Y; Lee JG; Buglass AJ; |
| Address: | "Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, South Korea" |
| DOI: | 10.1016/j.chroma.2013.04.056 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "A simultaneous multiple solid-phase microextraction-single shot-gas chromatography mass spectrometry (smSPME-ss-GC/MS) method has been developed for headspace analysis. Up to four fibers (50/30 mum DVB/CAR/PDMS) were used simultaneously for the extraction of aroma components from the headspace of a single sample chamber in order to increase sensitivity of aroma extraction. To avoid peak broadening and to maximize resolution, a simple cryofocusing technique was adopted during sequential thermal desorption of multiple SPME fibers prior to a 'single shot' chromatographic run. The method was developed and validated on a model flavor mixture, containing 81 known pure components. With the conditions of 10 min of incubation and 30 min of extraction at 50 degrees C, single, dual, triple and quadruple SPME extractions were compared. The increase in total peak area with increase in the number of fibers showed good linearity (R(2)=0.9917) and the mean precision was 12.0% (RSD) for the total peak sum, with quadruple simultaneous SPME extraction. Using a real sample such as commercial coffee granules, aroma profile analysis was conducted using single, dual, triple and quadruple SPME fibers. The increase in total peak intensity again showed good linearity with increase in the number of SPME fibers used (R(2)=0.9992) and the precision of quadruple SPME extraction was 9.9% (RSD) for the total peak sum" |
| Keywords: | Coffea/*chemistry Coffee/chemistry Gas Chromatography-Mass Spectrometry/*methods Odorants/analysis Plant Extracts/*analysis/*isolation & purification Solid Phase Microextraction/*methods Volatile Organic Compounds/*analysis/*isolation & purification; |
| Notes: | "MedlineLee, Changgook Lee, Younghoon Lee, Jae-Gon Buglass, Alan J eng Evaluation Study Research Support, Non-U.S. Gov't Netherlands 2013/05/16 J Chromatogr A. 2013 Jun 21; 1295:24-41. doi: 10.1016/j.chroma.2013.04.056. Epub 2013 Apr 24" |
