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Biol Chem

Title:		A conserved Gbeta binding (GBB) sequence motif in Ste20p/PAK family protein kinases
Author(s):		Leberer E; Dignard D; Thomas DY; Leeuw T;
Address:		"Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec"
Journal Title:		Biol Chem
Year:		2000
Volume:		381
Issue:		5-Jun
Page Number:		427 - 431
DOI:		10.1515/BC.2000.055
ISSN/ISBN:		1431-6730 (Print) 1431-6730 (Linking)
Abstract:		"Serine/threonine protein kinases of the Ste20p/PAK family are highly conserved from yeast to man. These protein kinases have been implicated in the signaling from heterotrimeric G proteins to mitogen-activated protein (MAP) kinase cascades and to cytoskeletal components such as myosin-I. In the yeast Saccharomyces cerevisiae, Ste20p is involved in transmitting the mating-pheromone signal from the betagamma-subunits of a heterotrimeric G protein to a downstream MAP kinase cascade. We have previously shown that binding of the G-protein beta-subunit (Gbeta) to a short binding site in the non-catalytic carboxy-terminal region of Ste20p is essential fortransmitting the pheromone signal. In this study, we searched protein sequence databases for sequences that are similar to the Gbeta binding site in Ste20p. We identified a sequence motif with the consensus sequence S S L phi P L I/V x phi phi beta (x: any residue; phi: A, I, L, S, or T; beta: basic residues) that is solely present in members of Ste20p/PAK family protein kinases. We propose that this sequence motif, which we have designated GBB (Gbeta binding) motif, is specifically responsible for binding of Gbeta to Ste20p/PAK protein kinases in response to activation of heterotrimeric G protein coupled receptors. Thus, the GBB motif is a novel type of signaling domain that serves to link protein kinases of the Ste20p/PAK family to G protein coupled receptors"
Keywords:		Amino Acid Sequence Binding Sites Heterotrimeric GTP-Binding Proteins/chemistry/*metabolism Humans Intracellular Signaling Peptides and Proteins MAP Kinase Kinase Kinases Molecular Sequence Data Protein Serine-Threonine Kinases/chemistry/*metabolism *Sacc;
Notes:		"MedlineLeberer, E Dignard, D Thomas, D Y Leeuw, T eng Research Support, Non-U.S. Gov't Review Germany 2000/08/11 Biol Chem. 2000 May-Jun; 381(5-6):427-31. doi: 10.1515/BC.2000.055"