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J Agric Food Chem

Title:		Characterization of volatiles and aroma-active compounds in honeybush (Cyclopia subternata) by GC-MS and GC-O analysis
Author(s):		le Roux M; Cronje JC; Burger BV; Joubert E;
Address:		"Department of Chemistry and Polymer Science, Stellenbosch University, Matieland, Stellenbosch, South Africa. mlr3@sun.ac.za"
Journal Title:		J Agric Food Chem
Year:		2012
Volume:		20120306
Issue:		10
Page Number:		2657 - 2664
DOI:		10.1021/jf2048383
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Volatile organic compounds (VOCs) in fermented honeybush, Cyclopia subternata, were sampled by means of a high-capacity headspace sample enrichment probe (SEP) and analyzed by gas chromatography-mass spectrometry (GC-MS). Stereochemistry was determined by means of enantioselective GC-MS with derivatized beta-cyclodextrin columns as chiral selectors. A total of 183 compounds, the majority of which are terpenoids (103; 56%), were identified by comparing their mass spectra and retention indices with those of reference compounds or tentatively identified by comparison with spectral library or literature data. Of these compounds, 37 were determined by gas chromatography-olfactometry (GC-O), using detection frequency (DF) and aroma extract dilution analysis (AEDA), to be odor-active (FD >/= 2). (E)-beta-Damascenone, (R/S)-linalool, (E)-beta-damascone, geraniol, (E)-beta-ionone, and (7E)-megastigma-5,7,9-trien-4-one were identified with the highest FD factors (>/=512). The odors of certain compounds, that is, (6E,8Z)-megastigma-4,6,8-trien-3-one, (6E,8E)-megastigma-4,6,8-trien-3-one, (7E)-megastigma-5,7,9-trien-4-one, 10-epi-gamma-eudesmol, epi-alpha-muurolol, and epi-alpha-cadinol, were perceived by GC-O assessors as typically honeybush-like"
Keywords:		"Chromatography, Gas/methods Cyclopia Plant/*chemistry Gas Chromatography-Mass Spectrometry/methods Plant Extracts/*analysis Volatile Organic Compounds/*analysis;"
Notes:		"MedlineLe Roux, Maritha Cronje, J Christel Burger, Barend V Joubert, Elizabeth eng Research Support, Non-U.S. Gov't 2012/03/08 J Agric Food Chem. 2012 Mar 14; 60(10):2657-64. doi: 10.1021/jf2048383. Epub 2012 Mar 6"