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Biochem Biophys Res Commun

Title:		Coil-to-helix transition and ligand release of Bombyx mori pheromone-binding protein
Author(s):		Lautenschlager C; Leal WS; Clardy J;
Address:		"Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA"
Journal Title:		Biochem Biophys Res Commun
Year:		2005
Volume:		335
Issue:		4
Page Number:		1044 - 1050
DOI:		10.1016/j.bbrc.2005.07.176
ISSN/ISBN:		0006-291X (Print) 0006-291X (Linking)
Abstract:		"The transport of hydrophobic insect pheromones through the aqueous medium surrounding their receptors is assisted by pheromone-binding proteins (PBPs). The protein from the silkworm moth Bombyx mori, BmorPBP, exhibits a pH-dependent conformational change postulated to trigger the release of the pheromone bombykol to its receptor. At low pH, an alpha-helix occupies the same binding pocket that houses the pheromone in the BmorPBP-bombykol complex at high pH. We have determined the crystal structure of apo BmorPBP at a resolution of 2.3 angstroms and pH 7.5, which has surprisingly a structure similar to the A-form. These data suggest that BmorPBP undergoes a ligand-dependent conformational change in addition to the previously described pH-dependent conformational change. Analysis of the alpha-helix occupying the binding pocket reveals an amphipathic helix with three acidic residues along one face that are conserved among lepidopteran PBPs and may be involved in a conformational transition of BmorPBP at the receptor membrane"
Keywords:		"Amino Acid Sequence Animals Binding Sites Bombyx/*metabolism Carrier Proteins/*analysis/*chemistry Computer Simulation Hydrogen-Ion Concentration Insect Proteins/*analysis/*chemistry Ligands *Models, Chemical *Models, Molecular Molecular Sequence Data Mot;"
Notes:		"MedlineLautenschlager, Catherine Leal, Walter S Clardy, Jon eng 1U01AI058267-01/AI/NIAID NIH HHS/ CA24487/CA/NCI NIH HHS/ CA59021/CA/NCI NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 2005/08/23 Biochem Biophys Res Commun. 2005 Oct 7; 335(4):1044-50. doi: 10.1016/j.bbrc.2005.07.176"