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Genetics

Title:		The fission yeast git5 gene encodes a Gbeta subunit required for glucose-triggered adenylate cyclase activation
Author(s):		Landry S; Pettit MT; Apolinario E; Hoffman CS;
Address:		"Department of Biology, Boston College, Chestnut Hill, MA 02467, USA"
Journal Title:		Genetics
Year:		2000
Volume:		154
Issue:		4
Page Number:		1463 - 1471
DOI:		10.1093/genetics/154.4.1463
ISSN/ISBN:		0016-6731 (Print) 0016-6731 (Linking)
Abstract:		"Fission yeast adenylate cyclase is activated by the gpa2 Galpha subunit of a heterotrimeric guanine-nucleotide binding protein (G protein). We show that the git5 gene, also required for this activation, encodes a Gbeta subunit. In contrast to another study, we show that git5 is not a negative regulator of the gpa1 Galpha involved in the pheromone response pathway. While 43% identical to mammalian Gbeta's, the git5 protein lacks the amino-terminal coiled-coil found in other Gbeta subunits, yet the gene possesses some of the coding capacity for this structure 5' to its ORF. Although both gpa2 (Galpha) and git5 (Gbeta) are required for adenylate cyclase activation, only gpa2 is needed to maintain basal cAMP levels. Strains bearing a git5 disruption are derepressed for fbp1 transcription and sexual development even while growing in a glucose-rich environment, although fbp1 derepression is half that observed in gpa2 deletion strains. Multicopy gpa2 partially suppresses the loss of git5, while the converse is not true. These data suggest that Gbeta is required for activation of adenylate cyclase either by promoting the activation of Galpha or by independently activating adenylate cyclase subsequent to Galpha stimulation as seen in type II mammalian adenylate cyclase activation"
Keywords:		"Adenylyl Cyclases/*metabolism Amino Acid Sequence Base Sequence Cloning, Molecular Codon Cyclic AMP/metabolism DNA Primers DNA, Recombinant Enzyme Activation *GTP-Binding Protein beta Subunits Glucose/*pharmacology Heterotrimeric GTP-Binding Proteins/*gen;"
Notes:		"MedlineLandry, S Pettit, M T Apolinario, E Hoffman, C S eng R01 GM046226-09/GM/NIGMS NIH HHS/ GM6226/GM/NIGMS NIH HHS/ R01 GM046226-11/GM/NIGMS NIH HHS/ R01 GM046226-12/GM/NIGMS NIH HHS/ R01 GM046226-10A1/GM/NIGMS NIH HHS/ R01 GM046226-13/GM/NIGMS NIH HHS/ R01 GM046226/GM/NIGMS NIH HHS/ R01 GM046226-13S1/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 2000/04/04 Genetics. 2000 Apr; 154(4):1463-71. doi: 10.1093/genetics/154.4.1463"