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Planta

Title:		Metabolic changes induced by Cuscuta campestris Yunck in the host species Artemisia campestris subsp. variabilis (Ten.) Greuter as a strategy for successful parasitisation
Author(s):		Landi M; Misra BB; Nocito FF; Lucchini G; Bruno L; Malara A; Abenavoli MR; Araniti F;
Address:		"Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124, Pisa, Italy. Independent Researcher, Pine-211, Raintree Park Dwaraka Krishna, Namburu, 522508, India. Dipartimento di Scienze Agrarie e Ambientali-Produzione, Territorio, Agroenergia, Universita degli Studi di Milano, Via Celoria 2, 20133, Milano, Italy. Dipartimento di Biologia, Ecologia e Scienzedella Terra (DiBEST), Universita della Calabria, 87036, Arcavacata di Rende, Cosenza, Italy. Dipartimento di Ingegneria Civile, dell'Energia, Dell'Ambiente e dei Materiali (DICEAM), Universita degli Studi 'Mediterranea' di Reggio Calabria, Loc. Feo di Vito, 89122, Reggio Calabria, Italy. Dipartimento AGRARIA, Universita degli Studi 'Mediterranea' di Reggio Calabria, localita Feo di Vito SNC, 89124, Reggio Calabria, Italy. Dipartimento di Scienze Agrarie e Ambientali-Produzione, Territorio, Agroenergia, Universita degli Studi di Milano, Via Celoria 2, 20133, Milano, Italy. Fabrizio.Araniti@unimi.it"
Journal Title:		Planta
Year:		2022
Volume:		20221115
Issue:		6
Page Number:		118 -
DOI:		10.1007/s00425-022-04025-8
ISSN/ISBN:		1432-2048 (Electronic) 0032-0935 (Print) 0032-0935 (Linking)
Abstract:		"C. campestris parasitisation increases internal host defences at the expense of environmentally directed ones in the host species A. campestris, thus limiting plant defence against progressive parasitisation. Cuscuta campestris Yunck is a holoparasitic species that parasitises wild species and crops. Among their hosts, Artemisia campestris subsp. variabilis (Ten.) Greuter is significantly affected in natural ecosystems. Limited information is available on the host recognition mechanism and there are no data on the interactions between these species and the effects on the primary and specialised metabolism in response to parasitisation. The research aims at evaluating the effect of host-parasite interactions, through a GC-MS untargeted metabolomic analysis, chlorophyll a fluorescence, ionomic and delta(13)C measurements, as well as volatile organic compound (VOC) fingerprint in A. campestris leaves collected in natural environment. C. campestris parasitisation altered plant water status, forcing stomatal opening, stimulating plant transpiration, and inducing physical damages to the host antenna complex, thus reducing the efficiency of its photosynthetic machinery. Untargeted-metabolomics analysis highlighted that the parasitisation significantly perturbed the amino acids and sugar metabolism, inducing an increase in the production of osmoprotectants, which generally accumulate in plants as a protective strategy against oxidative stress. Notably, VOCs analysis highlighted a reduction in sesquiterpenoids and an increase in monoterpenoids levels; involved in plant defence and host recognition, respectively. Moreover, C. campestris induced in the host a reduction in 3-hexenyl-acetate, a metabolite with known repellent activity against Cuscuta spp. We offer evidences that C. campestris parasitisation increases internal host defences via primary metabolites at the expense of more effective defensive compounds (secondary metabolites), thus limiting A. campestris defence against progressive parasitisation"
Keywords:		*Cuscuta/metabolism *Artemisia Ecosystem Chlorophyll A/metabolism Photosynthesis Allelopathy Field dodder Host recognition Metabolomics Parasitisation Plant communication Volatilome;
Notes:		"MedlineLandi, Marco Misra, Biswapriya B Nocito, Fabio Francesco Lucchini, Giorgio Bruno, Leonardo Malara, Angela Abenavoli, Maria Rosa Araniti, Fabrizio eng RTI2018-094716-B-100/Spanish National Plan for Scientific and Technical Research and Innovation/ Germany 2022/11/16 Planta. 2022 Nov 15; 256(6):118. doi: 10.1007/s00425-022-04025-8"