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Mol Cell Biol

Title:		Alpha-factor structural gene mutations in Saccharomyces cerevisiae: effects on alpha-factor production and mating
Author(s):		Kurjan J;
Address:
Journal Title:		Mol Cell Biol
Year:		1985
Volume:		5
Issue:		4
Page Number:		787 - 796
DOI:		10.1128/mcb.5.4.787-796.1985
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"The role of alpha-factor structural genes MF alpha 1 and MF alpha 2 in alpha-factor production and mating has been investigated by the construction of mf alpha 1 and mf alpha 2 mutations that totally eliminate gene function. An mf alpha 1 mutant in which the entire coding region is deleted shows a considerable decrease in alpha-factor production and a 75% decrease in mating. Mutations in mf alpha 2 have little or no effect on alpha-factor production or mating. The mf alpha 1 mf alpha 2 double mutants are completely defective in mating and alpha-factor production. These results indicate that at least one alpha-factor structural gene product is required for mating in MAT alpha cells, that MF alpha 1 is responsible for the majority of alpha-factor production, and that MF alpha 1 and MF alpha 2 are the only active alpha-factor genes"
Keywords:		"Base Sequence Cloning, Molecular Fungal Proteins/*genetics Genes Genes, Fungal Mating Factor Mutation Peptides/*genetics Reproduction Saccharomyces cerevisiae/*genetics;"
Notes:		"MedlineKurjan, J eng Research Support, U.S. Gov't, P.H.S. 1985/04/01 Mol Cell Biol. 1985 Apr; 5(4):787-96. doi: 10.1128/mcb.5.4.787-796.1985"