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Mol Cell Biol

Title:		AFR1 acts in conjunction with the alpha-factor receptor to promote morphogenesis and adaptation
Author(s):		Konopka JB;
Address:		"Department of Microbiology, State University of New York at Stony Brook 11794-5222"
Journal Title:		Mol Cell Biol
Year:		1993
Volume:		13
Issue:		11
Page Number:		6876 - 6888
DOI:		10.1128/mcb.13.11.6876-6888.1993
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"Mating pheromone receptors activate a G-protein signaling pathway that induces changes in transcription, cell division, and morphogenesis needed for the conjunction of Saccharomyces cerevisiae. The C terminus of the alpha-factor pheromone receptor functions in two complex processes, adaptation and morphogenesis. Adaptation to alpha-factor may occur through receptor desensitization, and alpha-factor-induced morphogenesis forms the conjugation bridge between mating cells. A plasmid overexpression strategy was used to isolate a new gene, AFR1, which acts together with the receptor C terminus to promote adaptation. The expression of AFR1 was highly induced by alpha-factor. Unexpectedly, cells lacking AFR1 showed a defect in alpha-factor-stimulated morphogenesis that was similar to the morphogenesis defect observed in cells producing C-terminally truncated alpha-factor receptors. In contrast, AFR1 overexpression resulted in longer projections of morphogenesis, which suggests that this gene may directly stimulate morphogenesis. These results indicate that AFR1 encodes a developmentally regulated function that coordinates both the regulation of receptor signaling and the induction of morphogenesis during conjugation"
Keywords:		"Acclimatization Amino Acid Sequence Base Sequence Cell Division DNA, Fungal/analysis/genetics Fungal Proteins/biosynthesis/genetics/*metabolism GTP-Binding Proteins/metabolism Gene Expression Regulation, Fungal/drug effects Genes, Fungal Genotype Kinetics;"
Notes:		"MedlineKonopka, J B eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1993/11/01 Mol Cell Biol. 1993 Nov; 13(11):6876-88. doi: 10.1128/mcb.13.11.6876-6888.1993"