| Title: | Dimethyl sulfoxide and ascarosides improve the growth and yields of entomopathogenic nematodes in liquid cultures |
| Author(s): | Kong X; Huang Z; Gu X; Cui Y; Li J; Han R; Jin Y; Cao L; |
| Address: | "Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China; Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, China. Yuxi Branch of Yunnan Tobacco Company, Engineering Center for Biological Control of Diseases and Pests in Tobacco Industry, Yuxi 653100, Yunnan Province, China. Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, China. Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang, China. Electronic address: gygjyl08@163.com. Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, China. Electronic address: caol@giz.gd.cn" |
| DOI: | 10.1016/j.jip.2022.107800 |
| ISSN/ISBN: | 1096-0805 (Electronic) 0022-2011 (Linking) |
| Abstract: | "Increasing the infective juvenile (IJ) yields of entomopathogenic nematodes in monoxenic culture systems would reduce their production cost for the market. Ascarosides act as universal nematode pheromones with developmental and behavioral effects of nematodes. Dimethyl sulfoxide (DMSO) is unexpectedly found to enhance the IJ yields of entomopathogenic nematodes on fortified nutrient broth plates. In this study, the influence of selected ascarosides (ascr#7, ascr#9 and ascr#11) and DMSO in three concentrations on the IJ yields of S. carpocapsae All and H. bacteriophora H06 in liquid culture flasks was determined, and the critical development parameters (IJ recovery rate, number of hermaphrodites, number of visible eggs in a hermaphrodite) were examined for H. bacteriophora H06. The results demonstrated that IJ yields were significantly improved in the liquid medium containing 0.01 % DMSO, and 0.02 nM ascr#11 for S. carpocapsae All, and 0.1 % and 0.01 % DMSO and 0.02 pM ascr#11 for H. bacteriophora H06 in proper concentrations. Furthermore, it was discovered that increased recovery rate, hermaphrodite numbers and eggs in the hermaphrodites may contribute to the improved IJ yields of H. bacteriophora H06 in DMSO-supplemented liquid medium. Compared with the control flasks, the IJ yields from the flasks containing 0.01 % DMSO were 15 % and 35 % higher for S. carpocapsae All and H. bacteriophora H06 respectively in 15 days. The cost for ascarosides and DMSO is almost negligible. The results would provide practical technology for low-cost commercial production of these nematodes for pest management program" |
| Keywords: | "Animals Dimethyl Sulfoxide *Nematoda Pest Control, Biological Pheromones *Rhabditida Ascarosides Entomopathogenic nematode Liquid culture Yield of infective juveniles;" |
| Notes: | "MedlineKong, Xiangxin Huang, Zhihua Gu, Xinghui Cui, Yonghe Li, Jiangzhou Han, Richou Jin, Yongling Cao, Li eng Research Support, Non-U.S. Gov't 2022/07/24 J Invertebr Pathol. 2022 Sep; 193:107800. doi: 10.1016/j.jip.2022.107800. Epub 2022 Jul 21" |
