| Title: | A comprehensive analysis of gene expression profiles in a yeast N-glycosylation mutant |
| Author(s): | Klebl B; Kozian D; Leberer E; Kukuruzinska MA; |
| Address: | "Aventis Pharma GmbH Deutschland, Aventis Center for Functional Genomics, Fraunhoferstrasse 13, D-82152 Martinsried, Germany" |
| Journal Title: | Biochem Biophys Res Commun |
| ISSN/ISBN: | 0006-291X (Print) 0006-291X (Linking) |
| Abstract: | "Although protein N-glycosylation is critical to many cell functions, its downstream targets remain largely unknown. In all eukaryotes, N-glycosylation utilizes the lipid-linked oligosaccharide (LLO) precursor, whose synthesis is initiated by the ALG7 gene. To elucidate the key signaling and metabolic events affected by N-glycosylation, we performed genomewide expression profiling of yeast cells carrying a hypomorphic allele of ALG7. DNA microarrays showed that of more than 97% of known or predicted yeast genes, 29 displayed increased expression while 23 were repressed in alg7 mutants. Changes in transcript abundance were observed for a and alpha mating-type genes, for genes functioning in several mitogen-activated protein kinase (MAPK) cascades, as well as in phosphate, amino acid, carbohydrate, mitochondrial and ATP metabolism. Therefore, DNA microarrays have revealed direct and indirect targets, including internal feedback loops, through which N-glycosylation affects signaling and metabolic activities and is functionally linked with cellular regulatory circuitry" |
| Keywords: | "3' Untranslated Regions Amino Acids/metabolism Carbohydrate Metabolism Gene Expression Profiling *Genes, Fungal *Genes, Mating Type, Fungal Glycosylation MAP Kinase Signaling System Mating Factor Mutation Oligonucleotide Array Sequence Analysis Peptides/g;" |
| Notes: | "MedlineKlebl, B Kozian, D Leberer, E Kukuruzinska, M A eng DE10183/DE/NIDCR NIH HHS/ Research Support, U.S. Gov't, P.H.S. 2001/08/25 Biochem Biophys Res Commun. 2001 Aug 31; 286(4):714-20. doi: 10.1006/bbrc.2001.5457" |
