| Title: | "Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3" |
| Author(s): | Kjaerulff S; Davey J; Nielsen O; |
| Address: | "Department of Genetics, University of Copenhagen, Denmark" |
| DOI: | 10.1128/mcb.14.6.3895-3905.1994 |
| ISSN/ISBN: | 0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking) |
| Abstract: | "We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells" |
| Keywords: | "Amino Acid Sequence Base Sequence Cloning, Molecular Conserved Sequence DNA Primers *Genes, Fungal Mating Factor Molecular Sequence Data Mutagenesis Open Reading Frames Peptide Biosynthesis Peptides/*genetics Pheromones/genetics Polymerase Chain Reaction;" |
| Notes: | "MedlineKjaerulff, S Davey, J Nielsen, O eng Comparative Study Research Support, Non-U.S. Gov't 1994/06/01 Mol Cell Biol. 1994 Jun; 14(6):3895-905. doi: 10.1128/mcb.14.6.3895-3905.1994" |
