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Eur Respir J

Title:		Breath analysis during one-lung ventilation in cancer patients
Author(s):		Kischkel S; Miekisch W; Fuchs P; Schubert JK;
Address:		"Department of Anaesthesiology and Intensive Care Medicine, University Rostock, Schillingallee 35, 18057 Rostock, Germany. sabine.kischkel@uni-rostock.de"
Journal Title:		Eur Respir J
Year:		2012
Volume:		20120120
Issue:		3
Page Number:		706 - 713
DOI:		10.1183/09031936.00125411
ISSN/ISBN:		1399-3003 (Electronic) 0903-1936 (Linking)
Abstract:		"Noninvasive breath analysis may provide valuable information for cancer recognition if disease-specific volatile biomarkers could be identified. In order to compare nondiseased and diseased tissue in vivo, this study took advantage of the special circumstances of one-lung ventilation (OLV) during lung-surgery. 15 cancer patients undergoing lung resection with OLV were enrolled. From each patient, alveolar breath samples were taken separately from healthy and diseased lungs before and after tumour resection. Volatile substances were pre-concentrated by means of solid-phase microextraction, and were separated, identified and quantified by means of gas chromatography-mass spectrometry. Different classes of volatile substances could be identified according to their concentration profiles. Due to prolonged fasting and activation of lipolysis, concentrations of endogenous acetone significantly increased during surgery. Exogenous substances, such as benzene or cyclohexanone, showed typical washout exhalation kinetics. Exhaled concentrations of potentially tumour associated substances, such as butane or pentane, were different for nondiseased and diseased lungs and decreased significantly after surgery. Separate analysis of volatile substances exhaled from healthy and diseased lungs in the same patient, together with thorough consideration of substance origins and exhalation kinetics offers unique opportunities of biomarker recognition and evaluation"
Keywords:		"Acetone/analysis Aged Benzene/analysis Biomarkers, Tumor/analysis Breath Tests Butanes/analysis Cyclohexanones/analysis Female Gas Chromatography-Mass Spectrometry/methods Humans Lung Neoplasms/*diagnosis Male Middle Aged *One-Lung Ventilation Pentanes/an;"
Notes:		"MedlineKischkel, Sabine Miekisch, Wolfram Fuchs, Patricia Schubert, Jochen K eng Clinical Trial Research Support, Non-U.S. Gov't England 2012/01/24 Eur Respir J. 2012 Sep; 40(3):706-13. doi: 10.1183/09031936.00125411. Epub 2012 Jan 20"