« Previous AbstractGlobal Plasma Profiling for Colorectal Cancer-Associated Volatile Organic Compounds: a Proof-of-Principle Study    Next AbstractImpact of Al doping on a hydrothermally synthesized beta-Ga(2)O(3) nanostructure for photocatalysis applications »

Genes (Basel)

Title:		Investigation of Mating Pheromone-Pheromone Receptor Specificity in Lentinula edodes
Author(s):		Kim S; Ha B; Kim M; Ro HS;
Address:		"Division of Applied Life Science and Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Korea"
Journal Title:		Genes (Basel)
Year:		2020
Volume:		20200504
Issue:		5
Page Number:		-
DOI:		10.3390/genes11050506
ISSN/ISBN:		2073-4425 (Electronic) 2073-4425 (Linking)
Abstract:		"The B mating-type locus of Lentinula edodes, a representative edible mushroom, is highly complex because of allelic variations in the mating pheromone receptors (RCBs) and the mating pheromones (PHBs) in both the Balpha and Bbeta subloci. The complexity of the B mating-type locus, five Balpha subloci with five alleles of RCB1 and nine PHBs and three Bbeta subloci with 3 alleles of RCB2 and five PHBs, has led us to investigate the specificity of the PHB-RCB interaction because the interaction plays a key role in non-self-recognition. In this study, the specificities of PHBs to RCB1-2 and RCB1-4 from the Balpha sublocus and RCB2-1 from the Bb sublocus were investigated using recombinant yeast strains generated by replacing STE2, an endogenous yeast mating pheromone receptor, with the L. edodesRCBs. Fourteen synthetic PHBs with C-terminal carboxymethylation but without farnesylation were added to the recombinant yeast cells and the PHB-RCB interaction was monitored by the expression of the FUS1 gene-a downstream gene of the yeast mating signal pathway. RCB1-2 (Balpha2) was activated by PHB1 (4.3-fold) and PHB2 (2.1-fold) from the Balpha1 sublocus and RCB1-4 (Balpha4) was activated by PHB5 (3.0-fold) and PHB6 (2.7-fold) from the Balpha2 sublocus and PHB13 (3.0-fold) from the Balpha5 sublocus. In particular, PHB3 from Bbeta2 and PHB9 from Bbeta3 showed strong activation of RCB2-1 of the Bbeta1 sublocus by 59-fold. The RCB-PHB interactions were confirmed in the monokaryotic S1-10 strain of L. edodes by showing increased expression of clp1, a downstream gene of the mating signal pathway and the occurrence of clamp connections after the treatment of PHBs. These results indicate that a single PHB can interact with a non-self RCB in a sublocus-specific manner for the activation of the mating pheromone signal pathways in L. edodes"
Keywords:		"Amino Acid Sequence Fungal Proteins/*genetics/metabolism Gene Expression Regulation, Fungal *Genes, Fungal Genes, Mating Type, Fungal/*genetics Mycelium/metabolism/ultrastructure Peptide Fragments/chemical synthesis/metabolism Pheromones/chemistry/metabol;"
Notes:		"MedlineKim, Sinil Ha, Byeongsuk Kim, Minseek Ro, Hyeon-Su eng Research Support, Non-U.S. Gov't Switzerland 2020/05/08 Genes (Basel). 2020 May 4; 11(5):506. doi: 10.3390/genes11050506"