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J Microbiol Biotechnol

Title:		Characterization of yakju brewed from glutinous rice and wild-type yeast strains isolated from nuruks
Author(s):		Kim HR; Kim JH; Bae DH; Ahn BH;
Address:		"Korean Alcoholic Beverage Research Center, Korea Food Research Institute, Seongnam 463-746, Korea"
Journal Title:		J Microbiol Biotechnol
Year:		2010
Volume:		20
Issue:		12
Page Number:		1702 - 1710
DOI:
ISSN/ISBN:		1738-8872 (Electronic) 1017-7825 (Linking)
Abstract:		"Korean traditional rice wines yakju and takju are generally brewed with nuruk as the source of the saccharogenic enzymes by natural fermentation. To improve the quality of Korean rice wine, the microorganisms in the nuruk need to be studied. The objective of this research was to improve the quality of Korean wine with the wild-type yeast strains isolated from the fermentation starter, nuruk. Only strain YA-6 showed high activity in 20% ethanol. Precipitation of Y89-5-3 was similar to that of very flocculent yeast (>80%) at 75.95%. Using 18S rRNA sequencing, all 10 strains were identified as Saccharomyces cerevisiae. Volatile compounds present in yakju were analyzed by gas chromatography-mass selective detector. The principal component analysis (PCA) of the volatile compounds grouped long-chain esters on the right side of the first principal component, PC1; these compounds were found in yakju that was made with strains YA-6, Y89-5-3, Y89-5- 2, Y90-9, and Y89-1-1. On the other side of PC1 were short-chain esters; these compounds were found in wines that were brewed with strains Y183-2, Y268-3, Y54-3, Y98-4, and Y88-4. Overall, the results indicated that using different wild-type yeast strains in the fermentation process significantly affects the chemical characteristics of the glutinous rice wine"
Keywords:		"Cluster Analysis DNA, Fungal/chemistry/genetics DNA, Ribosomal/chemistry/genetics Ethanol/metabolism/toxicity Gas Chromatography-Mass Spectrometry Genes, rRNA Molecular Sequence Data Oryza/*metabolism/*microbiology Phylogeny RNA, Fungal/genetics RNA, Ribo;"
Notes:		"MedlineKim, Hye Ryun Kim, Jae-Ho Bae, Dong-Hoon Ahn, Byung-Hak eng Research Support, Non-U.S. Gov't Korea (South) 2011/01/05 J Microbiol Biotechnol. 2010 Dec; 20(12):1702-10"