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Chemosphere

Title:		Evaluation of dispersion methods for enumeration of microorganisms from peat and activated carbon biofilters treating volatile organic compounds
Author(s):		Khammar N; Malhautier L; Degrange V; Lensi R; Fanlo JL;
Address:		"Laboratoire Genie de l'Environnement Industriel, Ecole des Mines d'Ales, Avenue de Clavieres 6, 30319 Ales Cedex, France"
Journal Title:		Chemosphere
Year:		2004
Volume:		54
Issue:		3
Page Number:		243 - 254
DOI:		10.1016/S0045-6535(03)00721-5
ISSN/ISBN:		0045-6535 (Print) 0045-6535 (Linking)
Abstract:		"To enumerate microorganisms having colonized biofilters treating volatile organic compounds, it is necessary firstly to evaluate dispersion methods. Crushing, shaking and sonication were then tested for the removal of microflora from biofilters packing materials (peat and activated carbon). Continuous or discontinuous procedures, and addition of glass beads had no effect on the number of microorganisms removed from peat particles. The duration of treatment also had no effect for shaking and crushing, but the number of microorganisms after 60 min of treatment with ultrasound was significantly higher than that obtained after 0.5 min. The comparison between these methods showed that crushing was the most efficient for the removal of microorganisms from both peat and activated carbon. The comparison between three chemical dispersion agents showed that 1% Na-pyrophosphate was less efficient, compared with 200 mM phosphate buffer or 1% Na-hexametaphosphate. To optimize the cultivation of microorganisms, three different agar media were compared. Tryptic soy agar tenfold diluted (TSA 1/10) was the most suitable medium for the culture of microflora from a peat biofilter. For the activated carbon biofilter, there was no significant difference between Luria Bertoni, TSA 1/10, and plate count agar. The optimized extraction and enumeration protocols were used to perform a quantitative characterization of microbial populations in an operating laboratory activated carbon biofilter and in two parallel peat biofilters"
Keywords:		"Air Pollution/*prevention & control *Biofilms Filtration/*instrumentation/methods Microscopy, Electron Organic Chemicals/analysis Soil/*analysis Ultrasonics;"
Notes:		"MedlineKhammar, Nadia Malhautier, Luc Degrange, Valerie Lensi, Robert Fanlo, Jean-Louis eng Comparative Study Research Support, Non-U.S. Gov't England 2003/10/25 Chemosphere. 2004 Jan; 54(3):243-54. doi: 10.1016/S0045-6535(03)00721-5"