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EMBO J

Title:		Four mating-type genes control sexual differentiation in the fission yeast
Author(s):		Kelly M; Burke J; Smith M; Klar A; Beach D;
Address:		"Cold Spring Harbor Laboratory, NY 11724"
Journal Title:		EMBO J
Year:		1988
Volume:		7
Issue:		5
Page Number:		1537 - 1547
DOI:		10.1002/j.1460-2075.1988.tb02973.x
ISSN/ISBN:		0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking)
Abstract:		"The mating-type region of fission yeast consists of three components, mat1, mat2-P and mat3-M, each separated by 15 kb. Cell-type is determined by the alternate allele present at mat1, either P in an h+ or M in an h- cell. mat2-P and mat3-M serve as donors of information that is transposed to mat1 during a switch of mating type. We have determined the nucleotide sequence of each component of mat. The P and M specific regions are 1104 and 1128 bp, respectively, and bounded by sequences common to each mating-type cassette (H1; 59 bp and H2; 135 bp). A third sequence is present at mat2-P and mat3-M but absent at mat1 (H3; 57 bp), and may be involved in transcriptional repression of these cassettes. mat1-P and mat1-M each encode two genes (Pc; 118 amino acids, Pi; 159 amino acids, Mc; 181 amino acids and Mi; 42 amino acids). Introduction of opal or frame-shift mutations into the open-reading-frame of each gene revealed that Pc and Mc are necessary and sufficient for mating and confer an h+ or h- mating type respectively. All four genes are required for meiotic competence in an h+/h- diploid. The transcription of each mat gene is strongly influenced by nutritional conditions and full induction was observed only in nitrogen-free medium. The predicted product of the Pi gene contains a region of homology with the homeobox sequence, suggesting that this gene encodes a DNA binding protein that directly regulates the expression of other genes"
Keywords:		"Amino Acid Sequence Base Sequence DNA, Fungal/genetics Gene Expression Regulation *Genes, Fungal Genes, Homeobox *Genes, Mating Type, Fungal Mating Factor Meiosis Molecular Sequence Data Peptides/*genetics Saccharomycetales/*genetics Schizosaccharomyces/*;"
Notes:		"MedlineKelly, M Burke, J Smith, M Klar, A Beach, D eng GM25678/GM/NIGMS NIH HHS/ GM34517/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. England 1988/05/01 EMBO J. 1988 May; 7(5):1537-47. doi: 10.1002/j.1460-2075.1988.tb02973.x"