| Title: | Identification and Molecular Characterization of Geranyl Diphosphate Synthase (GPPS) Genes in Wintersweet Flower |
| Author(s): | Kamran HM; Hussain SB; Junzhong S; Xiang L; Chen LQ; |
| Address: | "Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China. Southwest Engineering Research Center for Landscape Architecture (State Forestry and Grassland Administration), Southwest Forestry University, Kunming 650224, China" |
| ISSN/ISBN: | 2223-7747 (Print) 2223-7747 (Electronic) 2223-7747 (Linking) |
| Abstract: | "Geranyl diphosphate synthase (GPPS) is a plastid localized enzyme that catalyzes the biosynthesis of Geranyl diphosphate (GPP), which is a universal precursor of monoterpenes. Wintersweet (Chimonanthus praecox L.), a famous deciduous flowering shrub with a strong floral scent character, could have GPPS-like homologs that are involved in monoterpenes biosynthesis, but it remains unclear. In the present study, five full-length GPPS and geranylgeranyl diphosphate synthases (GGPPS) genes were identified in the wintersweet transcriptome database. The isolated cDNAs showed high protein sequence similarity with the other plants GPPS and GGPPS. The phylogenetic analysis further classified these cDNAs into four distinct clades, representing heterodimeric GPPS small subunits (SSU1 and SSU2), homodimeric GPPS, and GGPPS. Analysis of temporal expression revealed that all genes have the highest transcript level at the full-open flower stage. From tissue-specific expression analysis, CpGPPS.SSU1 and CpGGPPS1 were predominantly expressed in petal and flower, whereas CpGPPS.SSU2, GPPS, and GGPPS2 showed a constitutive expression. Additionally, the subcellular localization assay identified the chloroplast localization of SSUs and GGPPSs proteins, and the yeast two-hybrid assay showed that both CpGPPS.SSU1 and CpGPPS.SSU2 can interact with the GGPPS proteins. Taken together, these preliminary results suggest that the heterodimeric GPPS can regulate floral scent biosynthesis in wintersweet flower" |
| Keywords: | Ggpps Gpps floral fragrance wintersweet yeast two-hybrid assay; |
| Notes: | "PubMed-not-MEDLINEKamran, Hafiz Muhammad Hussain, Syed Bilal Junzhong, Shang Xiang, Lin Chen, Long-Qing eng 31572165/National Natural Science Foundation of China/ 000019/Thousand Talents Plan of Yunnan Province-High-End Foreign Experts/ Switzerland 2020/05/28 Plants (Basel). 2020 May 24; 9(5):666. doi: 10.3390/plants9050666" |
