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Med Sci Monit

Title:		Current medical research with the application of coupled techniques with mass spectrometry
Author(s):		Kaluzna-Czaplinska J;
Address:		"Department of Chemistry, Institute of General and Ecological Chemistry, Technical University of Lodz, Lodz, Poland. jkaluzna@p.lodz.pl"
Journal Title:		Med Sci Monit
Year:		2011
Volume:		17
Issue:		5
Page Number:		RA117 - RA123
DOI:		10.12659/msm.881756
ISSN/ISBN:		1643-3750 (Electronic) 1234-1010 (Print) 1234-1010 (Linking)
Abstract:		"The most effective methods of analysis of organic compounds in biological fluids are coupled chromatographic techniques. Capillary gas chromatography/mass spectrometry (GC-MS) allows the most efficient separation, identification and quantification of volatile metabolites in biological fluids. Liquid chromatography-mass spectrometry (LC-MS) is especially suitable for the analysis of non-volatile and/or thermally unstable compounds. A major drawback of liquid chromatography-mass spectrometry is that no standard spectral libraries such as NIST and Wiley for GC-MS are available to facilitate the identification of unknown compounds. Moreover, the identification of potential new compounds, especially new biomarkers in LC-MS, is much more challenging than in GC-MS. Capillary electrophoresis coupled with mass spectrometry (CE-MS) has been widely used to characterize metabolomes. Capillary electrophoresis is a powerful technique for the separation of charged metabolites, offering high analyte resolution. The advantages of CE-MS are applicability for hydrophilic metabolites, robust separation efficiency and short duration of analysis. This review provides an overview of current chromatographic methods--gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry--and their applications in current medical research. The focus is on the description of metabonomics research, strategies for biomarkers identification, medical diagnoses of diseases and research of drugs"
Keywords:		Biomedical Research/*methods Environmental Monitoring Humans Mass Spectrometry/*methods Metabolic Diseases/diagnosis/metabolism Metabolomics Neoplasms/therapy;
Notes:		"MedlineKaluzna-Czaplinska, Joanna eng Research Support, Non-U.S. Gov't Review 2011/04/29 Med Sci Monit. 2011 May; 17(5):RA117-23. doi: 10.12659/msm.881756"