| Title: | Effects of microbial volatile organic compounds on Ganoderma lucidum growth and ganoderic acids production in Co-v-cultures (volatile co-cultures) |
| Author(s): | Kalantari-Dehaghi S; Hatamian-Zarmi A; Ebrahimi-Hosseinzadeh B; Mokhtari-Hosseini ZB; Nojoki F; Hamedi J; Hosseinkhani S; |
| Address: | "a Department of Life Science Engineering, Faculty of New Science and Technology , University of Tehran , Tehran , Iran. b Department of Chemical Engineering, Faculty of Petroleum and Petrochemical Engineering , Hakim Sabzevari University , Sabzevar , Iran. c Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science , University of Tehran , Tehran , Iran. d Department of Biochemistry, Faculty of Biological Sciences , Tarbiat Modares University , Tehran , Iran" |
| DOI: | 10.1080/10826068.2018.1541809 |
| ISSN/ISBN: | 1532-2297 (Electronic) 1082-6068 (Linking) |
| Abstract: | "Co-v-culture (co-cultivations of physically separated microbes that only interact through the air) systems were designed to investigate the effects of microbial volatile organic compounds (mVOCs) from about 20 different microbes, on a medicinal fungus, Ganoderma lucidum. For more accuracy in co-cultivations, a novel synchronized cultivation approach was tested for culturing G. lucidum. The hyphal growth of G. lucidum and the content of its ganoderic acids (GAs) were measured. In almost all of the co-v-cultures, there was an inhibiting effect on hyphal growth and a promoting effect on GAs contents. In inducing GAs production, Bacillus cereus PTCC 1247 and Pseudomonas aeruginosa UTMC 1404 were the most effective ones, as, compared to control cultures, GAs content increased 2.8 fold. Comparing different co-v-cultivations demonstrated that the concentrations of mVOCs, oxygen, and carbon dioxide were the main players in co-v-cultures. No correlation was found between hyphal growth and GAs production. Strains of the same species imposed totally different effects on hyphal growth or GAs production. This study has investigated the effects of mVOCs on G. lucidum for the first time. Moreover, it suggests that co-v-cultivation may be a promising biotechnological approach to improve the production in G. lucidum" |
| Keywords: | Bacteria/metabolism Coculture Techniques/methods Ganoderma/*drug effects/*growth & development/metabolism Hyphae/drug effects/growth & development/metabolism Triterpenes/*metabolism Volatile Organic Compounds/*pharmacology Co-v-culture ganoderic acids mic; |
| Notes: | "MedlineKalantari-Dehaghi, Saeid Hatamian-Zarmi, Ashrafalsadat Ebrahimi-Hosseinzadeh, Bahman Mokhtari-Hosseini, Zahra-Beagom Nojoki, Fahimeh Hamedi, Javad Hosseinkhani, Saman eng England 2019/03/02 Prep Biochem Biotechnol. 2019; 49(3):286-297. doi: 10.1080/10826068.2018.1541809. Epub 2019 Mar 1" |
