| Title: | 2-Acetyl-1-pyrroline augmentation in scented indica rice (Oryza sativa L.) varieties through Delta(1)-pyrroline-5-carboxylate synthetase (P5CS) gene transformation |
| Author(s): | Kaikavoosi K; Kad TD; Zanan RL; Nadaf AB; |
| Address: | "Department of Botany, Savitribai Phule Pune University, Pune, 411007, India. Department of Botany, Savitribai Phule Pune University, Pune, 411007, India. abnadaf@unipune.ac.in" |
| DOI: | 10.1007/s12010-015-1827-4 |
| ISSN/ISBN: | 1559-0291 (Electronic) 0273-2289 (Linking) |
| Abstract: | "2-Acetyl-1-pyrroline (2AP) has been identified as a principal aroma compound in scented rice varieties. Delta(1)-Pyrroline-5-carboxylate synthetase (P5CS) gene is reported to regulate the proline synthesis in plants and acts as the precursor of 2AP. Two scented indica rice varieties, namely Ambemohar 157 and Indrayani, were subjected to Agrobacterium tumefaciens-mediated genetic transformation containing P5CS gene. Overexpression of P5CS led to a significant increase in proline, P5CS enzyme activity and 2AP levels in transgenic calli, vegetative plant parts, and seeds over control in both the varieties. 2AP level increased more than twofold in transgenic seeds in both varieties. This is the first report of enhancement in 2AP content through overexpression of using P5CS gene, indicating the role of proline as a precursor amino acid in the biosynthesis of 2AP in scented rice" |
| Keywords: | Agrobacterium/genetics Genetic Engineering/*methods Odorants/*analysis Oryza/enzymology/*genetics/*metabolism/physiology Phosphotransferases (Alcohol Group Acceptor)/*genetics/metabolism Proline/metabolism Pyrroles/*metabolism Regeneration Seeds/metabolis; |
| Notes: | "MedlineKaikavoosi, Kayghobad Kad, Trupti D Zanan, Rahul L Nadaf, Altafhusain B eng 2015/09/06 Appl Biochem Biotechnol. 2015 Dec; 177(7):1466-79. doi: 10.1007/s12010-015-1827-4. Epub 2015 Sep 4" |
