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Horm Behav

Title:		"Effects of DEHP on the ecdysteroid pathway, sexual behavior and offspring of the moth Spodoptera littoralis"
Author(s):		Aviles A; Cordeiro A; Maria A; Bozzolan F; Boulogne I; Dacher M; Goutte A; Alliot F; Maibeche M; Massot M; Siaussat D;
Address:		"Sorbonne Universite - Institut d'Ecologie et des Sciences de l'Environnement de Paris (iEES Paris) - Departement d'Ecologie Sensorielle, Campus Pierre et Marie Curie (UPMC), 75252 Paris Cedex 05, France. Sorbonne Universite - Institut d'Ecologie et des Sciences de l'Environnement de Paris (iEES Paris) - Departement d'Ecologie Sensorielle, Campus Pierre et Marie Curie (UPMC), 75252 Paris Cedex 05, France; UPRES-EA 4358 GlycoMev, Universite de Rouen, Rouen, France. Sorbonne Universite - Institut d'Ecologie et des Sciences de l'Environnement de Paris (iEES Paris) - Departement d'Ecologie Sensorielle, Campus Pierre et Marie Curie (UPMC), 75252 Paris Cedex 05, France; Sorbonne Universite - Institut d'Ecologie et des Sciences de l'Environnement de Paris (iEES Paris) - Departement d'Ecologie Sensorielle, Centre INRA, Batiment 1, Route de Saint Cyr, 78026 Versailles cedex, Versailles, France. Ecole Pratique des Hautes Etudes (EPHE), PSL. UMR 7619 METIS, Universite Pierre et Marie Curie (UPMC) - Sorbonne Universites, Paris, France. Sorbonne Universite - Institut d'Ecologie et des Sciences de l'Environnement de Paris (iEES Paris) - Departement d'Ecologie Sensorielle, Campus Pierre et Marie Curie (UPMC), 75252 Paris Cedex 05, France. Electronic address: david.siaussat@sorbonne-universite.fr"
Journal Title:		Horm Behav
Year:		2020
Volume:		20200710
Issue:
Page Number:		104808 -
DOI:		10.1016/j.yhbeh.2020.104808
ISSN/ISBN:		1095-6867 (Electronic) 0018-506X (Linking)
Abstract:		"Bis(2-ethylhexyl) phthalate (DEHP) is a widely produced plasticizer that is considered to act as an endocrine-disrupting chemical in vertebrates and invertebrates. Indeed, many studies have shown that DEHP alters hormonal levels, reproduction and behavior in vertebrates. Few studies have focused on the effects of DEHP on insects, although DEHP is found almost everywhere in their natural habitats, particularly in soils and plants. Here, we investigated the effects of DEHP on the sexual behavior and physiology of a pest insect, the noctuid moth Spodoptera littoralis. In this nocturnal species, olfaction is crucial for sexual behavior, and ecdysteroids at the antennal level have been shown to modulate sex pheromone detection by males. In the present study, larvae were fed food containing different DEHP concentrations, and DEHP concentrations were then measured in the adults (males and females). Hemolymphatic ecdysteroid concentrations, the antennal expression of genes involved in the ecdysteroid pathway (nuclear receptors EcR, USP, E75, and E78 and calmodulin) and sexual behavior were then investigated in adult males. The success and latency of mating as well as the hatching success were also studied in pairs consisting of one DEHP male and one uncontaminated female or one DEHP female and one uncontaminated male. We also studied the offspring produced from pairs involving contaminated females to test the transgenerational effect of DEHP. Our results showed the general downregulation of nuclear receptors and calmodulin gene expression associated with the higher concentrations of DEHP, suggesting peripheral olfactory disruption. We found some effects on male behavior but without an alteration of the mating rate. Effects on offspring mortality and developmental rates in the N?ª++?ª+1 generation were also found at the higher doses of DEHP. Taken together, the results of the study show for the first time that larval exposure to DEHP can induce delayed endocrine-disruptive effects in the adults of a terrestrial insect as well as effects on the next generation. To date, our study is also the first description of an impact of endocrine disrupter on olfaction in insects"
Keywords:		Animals Diethylhexyl Phthalate/*pharmacology Ecdysteroids/*metabolism Endocrine Disruptors/*pharmacology Female Larva/drug effects/metabolism Male Maternal Exposure/adverse effects Metabolic Networks and Pathways/drug effects/genetics Moths/drug effects/p;
Notes:		"MedlineAviles, Amandine Cordeiro, Alexandra Maria, Annick Bozzolan, Francoise Boulogne, Isabelle Dacher, Matthieu Goutte, Aurelie Alliot, Fabrice Maibeche, Martine Massot, Manuel Siaussat, David eng Research Support, Non-U.S. Gov't 2020/07/07 Horm Behav. 2020 Sep; 125:104808. doi: 10.1016/j.yhbeh.2020.104808. Epub 2020 Jul 10"