| Title: | Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation |
| Author(s): | Julio ADL; de Cassia Mourao Silva U; Medeiros JD; Morais DK; Dos Santos VL; |
| Address: | "Laboratory of Applied Microbiology, Microbiology Department, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Biosystems Informatics and Genomics Group, Rene Rachou Research Center/Fiocruz-MG, Belo Horizonte, MG, Brazil. Laboratory of Environmental Microbiology, Institute of Microbiology of the Czech Academy of Sciences - CAS, Prague, Czech Republic. Laboratory of Applied Microbiology, Microbiology Department, Institute of Biological Science, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. verabio@gmail.com" |
| DOI: | 10.1038/s41598-019-47921-y |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Metataxonomic approach was used to describe the bacterial community from a creosote-contaminated aquifer and to access the potential for in situ bioremediation of the polycyclic aromatic hydrocarbons (PAHs) by biostimulation. In general, the wells with higher PAH contamination had lower richness and diversity than others, using the Shannon and Simpson indices. By the principal coordinate analysis (PCoA) it was possible to observe the clustering of the bacterial community of most wells in response of the presence of PAH contamination. The significance analysis using edgeR package of the R program showed variation in the abundance of some Operational Taxonomic Units (OTUs) of contaminated wells compared to uncontaminated ones. Taxons enriched in the contaminated wells were correlated positively (p < 0.05) with the hydrocarbons, according to redundancy analysis (RDA). All these enriched taxa have been characterized as PAH degrading agents, such as the genus Comamonas, Geobacter, Hydrocarboniphaga, Anaerolinea and Desulfomonile. Additionally, it was possible to predict, with the PICRUSt program, a greater proportion of pathways and genes related to the degradation of PAHs in the wells with higher contamination levels. We conclude that the contaminants promoted the enrichment of several groups of degrading bacteria in the area, which strengthens the feasibility of applying biostimulation as an aquifer remediation strategy" |
| Keywords: | "Bacteria/*classification/*genetics Biodegradation, Environmental Cluster Analysis Creosote/*analysis *DNA Barcoding, Taxonomic Environmental Microbiology Groundwater/analysis/*chemistry/*microbiology Hydrocarbons/chemistry Volatile Organic Compounds *Wate;" |
| Notes: | "MedlineJulio, Aline Daniela Lopes de Cassia Mourao Silva, Ubiana Medeiros, Julliane Dutra Morais, Daniel Kumazawa Dos Santos, Vera Lucia eng Research Support, Non-U.S. Gov't England 2019/08/15 Sci Rep. 2019 Aug 13; 9(1):11731. doi: 10.1038/s41598-019-47921-y" |
