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EMBO J

Title:		Dominant-negative mutants of a yeast G-protein beta subunit identify two functional regions involved in pheromone signalling
Author(s):		Leberer E; Dignard D; Hougan L; Thomas DY; Whiteway M;
Address:		"Eukaryotic Genetics Group, National Research Council of Canada, Montreal, Quebec"
Journal Title:		EMBO J
Year:		1992
Volume:		11
Issue:		13
Page Number:		4805 - 4813
DOI:		10.1002/j.1460-2075.1992.tb05586.x
ISSN/ISBN:		0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking)
Abstract:		"The STE4 gene, which encodes the beta subunit of the mating response G-protein in the yeast Saccharomyces cerevisiae, was subjected to a saturation mutagenesis using 'doped' oligodeoxynucleotides. We employed a genetic screen to select dominant-negative STE4 mutants, which when overexpressed from the GAL1 promoter, interfered with the signalling function of the wild type protein. The identified inhibitory amino acid alterations define two small regions that are crucially involved in transmitting the mating signal from G beta to downstream components of the signalling pathway. These results underline the positive signalling role of yeast G beta and assign for the first time the positive signalling function of a G-protein beta subunit to specific structural features"
Keywords:		"Amino Acid Sequence *GTP-Binding Protein beta Subunits GTP-Binding Proteins/genetics/*metabolism Genes, Dominant *Heterotrimeric GTP-Binding Proteins Molecular Sequence Data *Mutation Pheromones/*metabolism Saccharomyces cerevisiae/genetics/*metabolism *S;"
Notes:		"MedlineLeberer, E Dignard, D Hougan, L Thomas, D Y Whiteway, M eng England 1992/12/01 EMBO J. 1992 Dec; 11(13):4805-13. doi: 10.1002/j.1460-2075.1992.tb05586.x"