| Title: | "Different roles suggested by sex-biased expression and pheromone binding affinity among three pheromone binding proteins in the pink rice borer, Sesamia inferens (Walker) (Lepidoptera: Noctuidae)" |
| Author(s): | Jin JY; Li ZQ; Zhang YN; Liu NY; Dong SL; |
| Address: | "Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; Suqian Entry-exit Inspection and Quarantine Bureau, Suqian, Jiangsu, China. Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: sldong@njau.edu.cn" |
| DOI: | 10.1016/j.jinsphys.2014.05.013 |
| ISSN/ISBN: | 1879-1611 (Electronic) 0022-1910 (Linking) |
| Abstract: | "Pheromone binding proteins (PBPs) are thought to bind and transport hydrophobic sex pheromone molecules across the aqueous sensillar lymph to specific pheromone receptors on the dendritic membrane of olfactory neurons. A maximum of 3 PBP genes have been consistently identified in noctuid species, and each of them shares high identity with its counterparts in other species within the family. The functionality differences of the 3 proteins are poorly understood. In the present study, 3 PBP cDNAs (SinfPBP1, 2, 3) were identified from the pink rice borer, Sesamia inferens, for the first time. The quantitative real-time PCR indicated that the 3 PBPs displayed similar temporal but very different sex related expression profiles. Expression of SinfPBP1 and SinfPBP2 were highly and moderately male biased, respectively, while SinfPBP3 was slightly female biased, as SinfPBPs were expressed at very different levels (PBP1>PBP2>>PBP3) in male antennae, but at similar levels in female antennae. Furthermore, the 3 SinfPBPs displayed different ligand binding profiles in fluorescence competitive binding assays. SinfPBP1 exhibited high and similar binding affinities to all 3 sex pheromone components (Ki=0.72-1.60 muM), while SinfPBP2 showed selective binding to the alcohol and aldehyde components (Ki=0.78-1.71 muM), and SinfPBP3 showed no obvious binding to the 3 sex pheromone components. The results suggest that SinfPBP1 plays a major role in the reception of female sex pheromones in S. inferens, while SinfPBP3 plays a least role (if any) and SinfPBP2 functions as a recognizer of alcohol and aldehyde components" |
| Keywords: | "Aging Amino Acid Sequence Animals Carrier Proteins/chemistry/*genetics/metabolism DNA, Complementary/genetics/metabolism Female Insect Proteins/chemistry/*genetics/metabolism Male Molecular Sequence Data Moths/*genetics/metabolism Organ Specificity Phylog;" |
| Notes: | "MedlineJin, Jun-Yan Li, Zhao-Qun Zhang, Ya-Nan Liu, Nai-Yong Dong, Shuang-Lin eng Research Support, Non-U.S. Gov't England 2014/05/28 J Insect Physiol. 2014 Jul; 66:71-9. doi: 10.1016/j.jinsphys.2014.05.013. Epub 2014 May 24" |
