| Title: | Functional analysis of odorant-binding proteins for the parasitic host location to implicate convergent evolution between the grain aphid and its parasitoid Aphidius gifuensis |
| Author(s): | Jiang X; Jiang J; Yu M; Zhang S; Qin Y; Xu Y; Francis F; Fan J; Chen J; |
| Address: | "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liege, Gembloux B-5030, Belgium. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Department of Entomology, MOA Key Laboratory for Monitoring and Environment-Friendly Control of Crop Pests, College of Plant Protection, China Agricultural University, Beijing 100193, PR China. Institute of Agricultural Environment & Resources, Yunnan Academy of Agricultural Science, Yunnan, Kunming 650205, PR China. Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liege, Gembloux B-5030, Belgium. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: jfan@ippcaas.cn. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: chenjulian@caas.cn" |
| DOI: | 10.1016/j.ijbiomac.2022.12.060 |
| ISSN/ISBN: | 1879-0003 (Electronic) 0141-8130 (Linking) |
| Abstract: | "(E)-beta-farnesene (EBF) is a typical and ecologically important infochemical in tri-trophic level interactions among plant-aphid-natural enemies. However, the molecular mechanisms by which parasitoids recognize and utilize EBF are unclear. In this study, we functionally characterized 8 AgifOBPs in Aphidifus gifuensis, one dominant endo-parasitoid of wheat aphid as well as peach aphid in China. Among which, AgifOBP6 was the only OBP upregulated by various doses of EBF, and it showed a strong binding affinity to EBF in vitro. The lack of homology between AgifOBP6 and EBF-binding proteins from aphids or from other aphid natural enemies supported that this was a convergent evolution among insects from different orders driven by EBF. Molecular docking of AgifOBP6 with EBF revealed key interacting residues and hydrophobic forces as the main forces. AgifOBP6 is widely expressed among various antennal sensilla. Furthermore, two bioassays indicated that trace EBF may promote the biological control efficiency of A. gifuensis, especially on winged aphids. In summary, this study reveals an OBP (AgifOBP6) that may play a leading role in aphid alarm pheromone detection by parasitoids and offers a new perspective on aphid biological control by using EBF. These results will improve our understanding of tri-trophic level interactions among plant-aphid-natural enemies" |
| Keywords: | Animals *Aphids Molecular Docking Simulation Odorants Pheromones/metabolism China (E)-beta-farnesene (EBF) Aphidifus gifuensis Convergent evolution Odorant-binding protein (OBPs) Olfactory plasticity; |
| Notes: | "MedlineJiang, Xin Jiang, Jun Yu, Miaomiao Zhang, Siyu Qin, Yaoguo Xu, Yun Francis, Frederic Fan, Jia Chen, Julian eng Netherlands 2022/12/13 Int J Biol Macromol. 2023 Jan 31; 226:510-524. doi: 10.1016/j.ijbiomac.2022.12.060. Epub 2022 Dec 9" |
