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Insect Biochem Mol Biol

Title:		Dissecting sex pheromone communication of Mythimna separata (Walker) in North China from receptor molecules and antennal lobes to behavior
Author(s):		Jiang NJ; Tang R; Wu H; Xu M; Ning C; Huang LQ; Wang CZ;
Address:		"State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, PR China. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, PR China. Electronic address: czwang@ioz.ac.cn"
Journal Title:		Insect Biochem Mol Biol
Year:		2019
Volume:		20190528
Issue:
Page Number:		103176 -
DOI:		10.1016/j.ibmb.2019.103176
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Linking)
Abstract:		"The Oriental armyworm, Mythimna separata, has been described to emit geographic population-specific sex pheromones, with either Z11-16:Ald or Z11-16:Ac as the major component. Using a comprehensive set of electrophysiological, behavioral, and genetic analyses, we study the sex pheromone communication of M. separata in North China from pheromone receptors and antennal lobe to behavior. GC-EAD results show that Z11-16:Ald is the only compound eliciting electrophysiological responses in pheromone gland extracts. Further in vivo optical imaging studies reveal that Z11-16:Ald activates the cumulus of the MGC and show dose-dependent responses. The wind tunnel tests demonstrate that Z11-16:Ald alone is sufficient to induce the entire sequence of male sexual behaviors. Transcriptome and q-PCR results show that MsepOR3 is specifically and abundantly expressed in male antennae. By using the Xenopus oocytes and two-electrode voltage-clamp recording, we finally validate that the oocytes expressing MsepOR3/ORco gave dose dependent responses to Z11-16:Ald. We suggest single Z11-16:Ald could be used for monitoring the population of M. separata in North China"
Keywords:		"Animals Arthropod Antennae/physiology China Electrophysiological Phenomena/drug effects Female Male Moths/*drug effects/genetics Oocytes Patch-Clamp Techniques Sex Attractants/*pharmacology Sexual Behavior, Animal/*drug effects Xenopus Behavior Glomerulus;"
Notes:		"MedlineJiang, Nan-Ji Tang, Rui Wu, Han Xu, Meng Ning, Chao Huang, Ling-Qiao Wang, Chen-Zhu eng Research Support, Non-U.S. Gov't England 2019/06/01 Insect Biochem Mol Biol. 2019 Aug; 111:103176. doi: 10.1016/j.ibmb.2019.103176. Epub 2019 May 28"