| Title: | Metabolic signatures of rhizobacteria-induced plant growth promotion |
| Author(s): | Jeon JS; Rybka D; Carreno-Quintero N; De Vos R; Raaijmakers JM; Etalo DW; |
| Address: | "Department of Microbial Ecology, Netherlands Institute of Ecology NIOO-KNAW, Wageningen, The Netherlands. Institute of Biology, Leiden University, Leiden, The Netherlands. KeyGene, Wageningen, The Netherlands. Wageningen Plant Research, Bioscience, Wageningen, The Netherlands. Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands" |
| ISSN/ISBN: | 1365-3040 (Electronic) 0140-7791 (Linking) |
| Abstract: | "Various root-colonizing bacterial species can promote plant growth and trigger systemic resistance against aboveground leaf pathogens and herbivore insects. To date, the underlying metabolic signatures of these rhizobacteria-induced plant phenotypes are poorly understood. To identify core metabolic pathways that are targeted by growth-promoting rhizobacteria, we used combinations of three plant species and three rhizobacterial species and interrogated plant shoot chemistry by untargeted metabolomics. A substantial part (50%-64%) of the metabolites detected in plant shoot tissue was differentially affected by the rhizobacteria. Among others, the phenylpropanoid pathway was targeted by the rhizobacteria in each of the three plant species. Differential regulation of the various branches of the phenylpropanoid pathways showed an association with either plant growth promotion or growth reduction. Overall, suppression of flavonoid biosynthesis was associated with growth promotion, while growth reduction showed elevated levels of flavonoids. Subsequent assays with 12 Arabidopsis flavonoid biosynthetic mutants revealed that the proanthocyanidin branch plays an essential role in rhizobacteria-mediated growth promotion. Our study also showed that a number of pharmaceutically and nutritionally relevant metabolites in the plant shoot were significantly increased by rhizobacterial treatment, providing new avenues to use rhizobacteria to tilt plant metabolism towards the biosynthesis of valuable natural plant products" |
| Keywords: | *Arabidopsis/genetics Flavonoids/metabolism Herbivory Plant Development Plant Roots/microbiology Plant Shoots bacteria-plant interactions beneficial rhizobacteria flavonoids high-value natural products metabolic alterations phenylpropanoids plant metabolo; |
| Notes: | "MedlineJeon, Je-Seung Rybka, Dominika Carreno-Quintero, Natalia De Vos, Ric Raaijmakers, Jos M Etalo, Desalegn W eng Research Support, Non-U.S. Gov't 2022/06/26 Plant Cell Environ. 2022 Oct; 45(10):3086-3099. doi: 10.1111/pce.14385. Epub 2022 Jul 4" |
