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Nat Commun

Title:		Engineered cell differentiation and sexual reproduction in probiotic and mating yeasts
Author(s):		Jensen ED; Deichmann M; Ma X; Vilandt RU; Schiesaro G; Rojek MB; Lengger B; Eliasson L; Vento JM; Durmusoglu D; Hovmand SP; Al'Abri I; Zhang J; Crook N; Jensen MK;
Address:		"Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark. emdaje@biosustain.dtu.dk. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark. Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27695, USA. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs, Lyngby, Denmark. mije@biosustain.dtu.dk"
Journal Title:		Nat Commun
Year:		2022
Volume:		20221019
Issue:		1
Page Number:		6201 -
DOI:		10.1038/s41467-022-33961-y
ISSN/ISBN:		2041-1723 (Electronic) 2041-1723 (Linking)
Abstract:		"G protein-coupled receptors (GPCRs) enable cells to sense environmental cues and are indispensable for coordinating vital processes including quorum sensing, proliferation, and sexual reproduction. GPCRs comprise the largest class of cell surface receptors in eukaryotes, and for more than three decades the pheromone-induced mating pathway in baker's yeast Saccharomyces cerevisiae has served as a model for studying heterologous GPCRs (hGPCRs). Here we report transcriptome profiles following mating pathway activation in native and hGPCR-signaling yeast and use a model-guided approach to correlate gene expression to morphological changes. From this we demonstrate mating between haploid cells armed with hGPCRs and endogenous biosynthesis of their cognate ligands. Furthermore, we devise a ligand-free screening strategy for hGPCR compatibility with the yeast mating pathway and enable hGPCR-signaling in the probiotic yeast Saccharomyces boulardii. Combined, our findings enable new means to study mating, hGPCR-signaling, and cell-cell communication in a model eukaryote and yeast probiotics"
Keywords:		"*Saccharomyces cerevisiae/metabolism Reproduction/genetics Receptors, G-Protein-Coupled/metabolism Pheromones/metabolism Receptors, Cell Surface/metabolism Cell Differentiation Cell Communication *Probiotics Ligands;"
Notes:		"MedlineJensen, Emil D Deichmann, Marcus Ma, Xin Vilandt, Rikke U Schiesaro, Giovanni Rojek, Marie B Lengger, Bettina Eliasson, Line Vento, Justin M Durmusoglu, Deniz Hovmand, Sandie P Al'Abri, Ibrahim Zhang, Jie Crook, Nathan Jensen, Michael K eng Research Support, Non-U.S. Gov't England 2022/10/20 Nat Commun. 2022 Oct 19; 13(1):6201. doi: 10.1038/s41467-022-33961-y"