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Cell

Title:		Down regulation of the alpha-factor pheromone receptor in S. cerevisiae
Author(s):		Jenness DD; Spatrick P;
Address:
Journal Title:		Cell
Year:		1986
Volume:		46
Issue:		3
Page Number:		345 - 353
DOI:		10.1016/0092-8674(86)90655-0
ISSN/ISBN:		0092-8674 (Print) 0092-8674 (Linking)
Abstract:		"The peptide pheromone, alpha-factor, was found to elicit down regulation of receptor sites on yeast a cell targets. Cellular uptake of alpha-factor accompanied the loss of receptor sites. Receptor-deficient a cells bearing a deletion of the STE2 gene were unable to internalize alpha-factor. Cultures were found to reaccumulate receptor sites following the initial period of down regulation; reaccumulation was dependent upon protein synthesis. Pheromone-resistant mutants, ste4-3 and ste5-3, retained the ability to down regulate receptors but failed to show reaccumulation. Our results suggest that alpha-factor-receptor complexes enter the cell by receptor-mediated endocytosis and that receptors are continuously lost and resynthesized in the presence of alpha-factor. We found no reduction of alpha-factor binding capacity in a cell cultures that had adapted to alpha-factor"
Keywords:		"Adaptation, Physiological Endocytosis Fungal Proteins/biosynthesis *Genes, Fungal Mating Factor Peptides/metabolism/*pharmacology Receptors, Cell Surface/genetics/*metabolism Receptors, Mating Factor *Receptors, Peptide Saccharomyces cerevisiae/drug effec;"
Notes:		"MedlineJenness, D D Spatrick, P eng GM34719/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1986/08/01 Cell. 1986 Aug 1; 46(3):345-53. doi: 10.1016/0092-8674(86)90655-0"