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Metabolites

Title:		Dependence of the Staphylococcal Volatilome Composition on Microbial Nutrition
Author(s):		Jenkins CL; Bean HD;
Address:		"School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA. Center for Fundamental and Applied Microbiomics, The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"
Journal Title:		Metabolites
Year:		2020
Volume:		20200827
Issue:		9
Page Number:		-
DOI:		10.3390/metabo10090347
ISSN/ISBN:		2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking)
Abstract:		"In vitro cultivation of staphylococci is fundamental to both clinical and research microbiology, but few studies, to-date, have investigated how the differences in rich media can influence the volatilome of cultivated bacteria. The objective of this study was to determine the influence of rich media composition on the chemical characteristics of the volatilomes of Staphylococcus aureus and Staphylococcus epidermidis. S. aureus (ATCC 12600) and S. epidermidis (ATCC 12228) were cultured in triplicate in four rich complex media (brain heart infusion (BHI), lysogeny broth (LB), Mueller Hinton broth (MHB), and tryptic soy broth (TSB)), and the volatile metabolites produced by each culture were analyzed using headspace solid-phase microextraction combined with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (HS-SPME-GCxGC-TOFMS). When comparing the chemical compositions of the staph volatilomes by the presence versus absence of volatiles produced in each medium, we observed few differences. However, when the relative abundances of volatiles were included in the analyses, we observed that culturing staph in media containing free glucose (BHI and TSB) resulted in volatilomes dominated by acids and esters (67%). The low-glucose media (LB and MHB) produced ketones in greatest relative abundances, but the volatilome compositions in these two media were highly dissimilar. We conclude that the staphylococcal volatilome is strongly influenced by the nutritional composition of the growth medium, especially the availability of free glucose, which is much more evident when the relative abundances of the volatiles are analyzed, compared to the presence versus absence"
Keywords:		GCxGC-TOFMS Staphylococcus aureus Staphylococcus epidermidis catabolite repression control volatile organic compounds;
Notes:		"PubMed-not-MEDLINEJenkins, Carrie L Bean, Heather D eng R56 HL139846/HL/NHLBI NIH HHS/ Hill18A0-CI/Cystic Fibrosis Foundation/ Switzerland 2020/09/02 Metabolites. 2020 Aug 27; 10(9):347. doi: 10.3390/metabo10090347"