| Title: | Neurospora crassa fmf-1 encodes the homologue of the Schizosaccharomyces pombe Ste11p regulator of sexual development |
| Author(s): | Iyer SV; Ramakrishnan M; Kasbekar DP; |
| Address: | "Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India" |
| DOI: | 10.1007/s12041-009-0005-2 |
| ISSN/ISBN: | 0973-7731 (Electronic) 0022-1333 (Linking) |
| Abstract: | "The Neurospora crassa fmf-1 mutation exerts an unusual 'perithecium-dominant' developmental arrest; fmf-1 x fmf-1+ cross becomes arrested in perithecial development regardless of whether the mutant participates in the cross as the male or female parent. We localized fmf-1 to the LG IL genome segment between the centromere-proximal breakpoint of the chromosome segment duplication Dp(IL)39311 and the centromere. By mapping crossovers with respect to RFLP markers in this region we further localized fmf-1 to an approximately 34-kb-genome segment. Partial sequencing of this segment revealed a point mutation in the gene NCU 09387.1, a homologue of the Schizosaccharomyces pombe ste11+ regulator of sexual development. The fmf-1 mutation did not complement a NCU 09387.1 deletion mutation, and transformation with wild-type NCU 09387.1 complemented fmf-1. S. pombe Ste11 protein (Ste11p) is a transcription factor required for sexual differentiation and for the expression of genes required for mating pheromone signalling in matP and matM cells. If FMF-1 also plays a corresponding role in mating pheromone signalling in Neurospora, then protoperithecia in an fmf-1 x fmf-1+ cross would be unable to either send or receive sexual differentiation signals and thus become arrested in development" |
| Keywords: | "Fungal Proteins/*genetics/metabolism Gene Expression Regulation, Fungal Genome, Fungal Models, Genetic Mutation Neurospora crassa/*genetics Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins/genetics Transcription Factors/*genetics/metabolism;" |
| Notes: | "MedlineIyer, Srividhya V Ramakrishnan, Mukund Kasbekar, Durgadas P eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. India 2009/05/07 J Genet. 2009 Apr; 88(1):33-9. doi: 10.1007/s12041-009-0005-2" |
