| Title: | Electrical stimulation disrupts biofilms in a human wound model and reveals the potential for monitoring treatment response with volatile biomarkers |
| Author(s): | Ashrafi M; Novak-Frazer L; Morris J; Baguneid M; Rautemaa-Richardson R; Bayat A; |
| Address: | "Plastic & Reconstructive Surgery Research, Division of Musculoskeletal & Dermatological Sciences, School of Biological Sciences, University of Manchester, Manchester, United Kingdom. Manchester University NHS Foundation Trust, Wythenshawe Hospital, Manchester, United Kingdom. Bioengineering Group, School of Materials, University of Manchester, Manchester, United Kingdom. Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, The University of Manchester and Manchester University NHS Foundation Trust, Manchester, United Kingdom. Honorary Reader in Medical Statistics, Manchester University NHS Foundation Trust, Wythenshawe Hospital, Manchester, United Kingdom" |
| ISSN/ISBN: | 1524-475X (Electronic) 1067-1927 (Linking) |
| Abstract: | "Management of biofilm infections relies on time-consuming laboratory techniques and monitoring treatment by subjective clinical evaluations. Due to these limitations, there is a need to explore alternative strategies. The aims of this study were to assess the feasibility of using volatile organic compound (VOC) biomarkers to monitor treatment response and measure anti-biofilm efficacy of electrical stimulation (ES) in vitro and in human cutaneous wound biofilm models. Staphylococcus aureus (MSSA) and Pseudomonas aeruginosa (PA) biofilms were exposed to ES, ciprofloxacin, or both, with efficacy assessed and quantified by fluorescence staining, enumeration, metabolic assays, and biomass quantification; VOCs were measured by gas chromatography-mass spectrometry. In vitro MSSA and PA and ex vivo PA biofilms exposed to ES showed significantly reduced bacterial viability, metabolic activity, and biomass compared to controls (p < 0.05). There was significant variation in the relative abundance of VOCs in in vitro MSSA and PA and in ex vivo PA biofilms exposed to ES and antibiotic (p < 0.05). 2-methyl-1-propanol was associated with MSSA viability (R = 0.93, p < 0.05), biomass (R = 0.97, p < 0.05), and metabolic activity (R = 0.93, p < 0.05) and 3-methyl-1-butanol was associated with PA biomass (R = 0.93, p < 0.05). We showed that ES and VOC biomarkers are possible options for alternative nonpharmacological antimicrobial management of biofilms and noninvasive monitoring of wound infection treatment responses, respectively" |
| Keywords: | "Biofilms/drug effects/*growth & development Biomarkers/analysis Cells, Cultured *Electric Stimulation Gas Chromatography-Mass Spectrometry Humans Microbial Sensitivity Tests Pseudomonas Infections/*microbiology Pseudomonas aeruginosa/drug effects Staphylo;" |
| Notes: | "MedlineAshrafi, Mohammed Novak-Frazer, Lilyann Morris, Julie Baguneid, Mohamed Rautemaa-Richardson, Riina Bayat, Ardeshir eng 2018/10/16 Wound Repair Regen. 2019 Jan; 27(1):5-18. doi: 10.1111/wrr.12679. Epub 2018 Nov 21" |
