| Title: | Gqalpha-linked phospholipase Cbeta1 and phospholipase Cgamma are essential components of the pheromone biosynthesis activating neuropeptide (PBAN) signal transduction cascade |
| Author(s): | Hull JJ; Lee JM; Matsumoto S; |
| Address: | "Molecular Entomology Laboratory, RIKEN Advanced Science Institute, Wako, Saitama, Japan. Joe.Hull@ars.usda.gov" |
| DOI: | 10.1111/j.1365-2583.2010.01013.x |
| ISSN/ISBN: | 1365-2583 (Electronic) 0962-1075 (Linking) |
| Abstract: | "Sex pheromone production for most moths is regulated by pheromone biosynthesis activating neuropeptide (PBAN). In Bombyx mori, PBAN binding triggers the opening of store-operated Ca(2+) channels, suggesting the involvement of a receptor-activated phospholipase C (PLC). In this study, we found that PLC inhibitors U73122 and compound 48/80 reduced sex pheromone production and that intracellular levels of (3)H-inositol phosphate species increased following PBAN stimulation. In addition, we amplified cDNAs from pheromone glands corresponding to PLCbeta1, PLCbeta4, PLCgamma and two G protein alpha subunits, Go and Gq. In vivo RNA interference-mediated knockdown analyses revealed that BmPLCbeta1, BmGq1, and unexpectedly, BmPLCgamma, are part of the PBAN signal transduction cascade" |
| Keywords: | "Amino Acid Sequence Animals Blotting, Western Bombyx/drug effects/*enzymology Enzyme Inhibitors/pharmacology Fatty Alcohols/metabolism Female GTP-Binding Protein alpha Subunits, Gq-G11/*metabolism Gene Expression Regulation, Enzymologic Models, Biological;" |
| Notes: | "MedlineHull, J J Lee, J M Matsumoto, S eng Research Support, Non-U.S. Gov't England 2010/06/16 Insect Mol Biol. 2010 Aug; 19(4):553-66. doi: 10.1111/j.1365-2583.2010.01013.x. Epub 2010 Jun 10" |
