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Bioprocess Biosyst Eng

Title:		Green ultrasonication-assisted extraction of microalgae Chlorella sp. for polysaturated fatty acid (PUFA) rich lipid extract using alternative solvent mixture
Author(s):		Hui GT; Meng TK; Kassim MA;
Address:		"Division of Bioprocess Technology, School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia. Division of Bioprocess Technology, School of Industrial Technology, Universiti Sains Malaysia, 11800, Penang, Malaysia. asyrafkassim@usm.my"
Journal Title:		Bioprocess Biosyst Eng
Year:		2023
Volume:		20230814
Issue:		10
Page Number:		1499 - 1512
DOI:		10.1007/s00449-023-02917-x
ISSN/ISBN:		1615-7605 (Electronic) 1615-7591 (Linking)
Abstract:		"Conventionally, microalgal lipid extraction uses volatile organic compounds as an extraction solvent. However, these solvents are harmful to human and environmental health. Therefore, this study evaluated the feasibility of alternative green solvents, namely, ethanol, dimethyl carbonate (DMC), cyclopentyl methyl ether (CPME), and 2-methyltetrahydrofuran (2-MeTHF) in lipid extraction from Chlorella sp. via ultrasound-assisted extraction (UAE). This study indicated that extraction parameters, such as ethanol-to-2-MeTHF ratio, solvent-to-biomass ratio, temperature, and time, significantly affected the crude lipid yield (P < 0.05). The highest crude lipid yield of 25.05 +/- 0.924% was achieved using ethanol-2-MeTHF mixture (2:1, v/v) with a solvent-to-biomass ratio of 20:1 (v/w) at 60 degrees C for 25 min accompanying 100 W and 40 kHz. Ethanol-2-MeTHF-extracted lipids showed dominance in linoleic acid, alpha-linolenic acid, and palmitic acid. Overall this findings supported UAE using ethanol and 2-MeTHF as extraction solvents is a promising green alternative to conventional solvent extraction of lipids from microalgae"
Keywords:		Humans Fatty Acids Solvents *Chlorella *Microalgae Ethanol Biomass Chlorella sp.Extraction Green solvent Microalgae Pufa;
Notes:		"MedlineHui, Goh Ting Meng, Tan Kean Kassim, Mohd Asyraf eng USM Grant 304.PTEKIND.6501151.N132/USM Grant/ Germany 2023/08/15 Bioprocess Biosyst Eng. 2023 Oct; 46(10):1499-1512. doi: 10.1007/s00449-023-02917-x. Epub 2023 Aug 14"