| Title: | [Determination of volatile organic compounds in lung cancer cell lines and lung cancer tissue] |
| Author(s): | Hu YJ; Qiu YH; Chen EG; Ying KJ; Yu J; Wang P; |
| Address: | "Department of Pulmonary Medicine, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China" |
| Journal Title: | Zhejiang Da Xue Xue Bao Yi Xue Ban |
| DOI: | 10.3785/j.issn.1008-9292.2010.03.011 |
| ISSN/ISBN: | 1008-9292 (Print) 1008-9292 (Linking) |
| Abstract: | "OBJECTIVE: To identify the volatile organic compounds (VOCs) in lung cancer tissue and lung cancer cell lines. METHODS: The lung cancer tissue samples from 18 patients were cultured and 4 lung cell lines (A549, NCI-H446, SK-MES-1, BEAS-2B) were also included in the study. Air samples in the headspace of culture flasks were analyzed for VOCs with solid-phase micro-extraction and gas chromatography-mass spectroscopy technique (SPME-GC/MS). RESULT: Two kinds of VOCs 2-pentadecanone and nonadecane were detected in lung cancer cell lines A549, NCI-H446 and SK-MES-1. The concentration of 2-pentadecanone were (1.382 + or -0.171) X 10(-5)mg/L, (1.681 + or - 0.190) X 10(-4)mg/L and (2.835 + or - 0.401) X 10(-6)mg/L, respectively; the concentrations of nonadecane were (8.382 + or - 0.606 ) X 10(-6)mg/L, (1.845 + or - 0.130) X 10(-5)mg/L and (6.220 + or - 0.362) X 10(-6)mg/L), respectively. The eicosane was detected in A549 and NCI-H446 with the concentration of (8.313 + or - 1.130) X 10(-6)mg/L and (1.020 + or - 0.141) X 10(-5)mg/L), respectively. All the 3 VOCs were not detected in cell line BEAS-2B. The concentrations of 12 VOCs including decane, 2- pentadecanone, nonadecane and eicosane were high in 18 lung cancer tissue samples; the concentrations of 2-pentadecanone were 5.421 X 10(-6)mg/L-3.621 X 10(-5)mg/L,those of nonadecane were 5.805 X 10(-6)mg/L-1.830 X 10(-5)mg/L, those of eicosane were 2.730 X 10(-6)mg/L-2.343 X 10(-5)mg/L. There were no differences of VOCs levels among patients with different cancer differentiation (P>0.05). The concentration of eicosane in the non-squamous carcinoma was higher than that in squamous carcinoma, the same results were confirmed in the lung cancer cell lines. CONCLUSION: This study has identified VOCs produced by lung cancer tissue, which may support to use breath test as a complementary noninvasive diagnostic method for lung cancer" |
| Keywords: | "Adult Aged Alkanes/metabolism Biomarkers, Tumor/*metabolism Cell Line, Tumor Female Humans Lung Neoplasms/diagnosis/*metabolism Male Middle Aged Tumor Cells, Cultured Volatile Organic Compounds/*metabolism;" |
| Notes: | "MedlineHu, Yan-jie Qiu, Yuan-hua Chen, En-guo Ying, Ke-jing Yu, Jin Wang, Ping chi English Abstract Research Support, Non-U.S. Gov't China 2010/06/15 Zhejiang Da Xue Xue Bao Yi Xue Ban. 2010 May; 39(3):278-84. doi: 10.3785/j.issn.1008-9292.2010.03.011" |
