| Title: | "OBP2 in the Midlegs of the Male Bactrocera dorsalis Is Involved in the Perception of the Female-Biased Sex Pheromone 4-Allyl-2,6-dimethoxyphenol" |
| Author(s): | Hu L; Chen B; Liu K; Yu G; Chen Y; Dai J; Zhao X; Zhong G; Zhang Y; Shen J; |
| Address: | "Institute of Plant Health, ZhongKai University of Agriculture and Engineering, 510225 Guangzhou, Guangdong, China. Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou, Guangdong China. College of Light industry and Food Science, ZhongKai University of Agriculture and Engineering, Guangzhou, Guangdong China. Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, China. Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081 Beijing, China" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "Short-range semiochemicals convey individual-specific information, which is important for final successful courtship and copulation. In this study, an electroantennography (EAG) instrument was used to determine the legs of male Bactrocera dorsalis that might participate in the perception of 4-allyl-2,6-dimethoxyphenol (4-DMP), a female-biased cuticular hydrocarbon (CHC) component. By performing comparative RNA-seq analysis, nine differentially expressed OBPs between 4-DMP-stimulated (M(4-DMP)) and unstimulated (M) male flies were screened out. Among the four downregulated OBPs, BdorOBP2 exhibited the strongest binding to 4-DMP than BdorOBP4, BdorOBP19a, and BdorOBP56h. Functional analyses confirmed that the reduction in transcript abundance of BdorOBP2 led to a significant decrease in behavioral responses of male flies to 4-DMP. In silico simulation revealed dramatic changes in the key residues and conformation between the two complexes, implying that BdorOBP2 might activate different receptors after binding to 4-DMP or methyl eugenol (ME). The results of this study suggest that BdorOBP2 mediates behavioral responses to 4-DMP and could be a promising molecular target for strategies of pest control" |
| Keywords: | "Animals Eugenol/*analogs & derivatives/chemistry/metabolism Female Insect Proteins/chemistry/genetics/*metabolism Male Receptors, Odorant/chemistry/genetics/*metabolism Sex Attractants/chemistry/*metabolism Tephritidae/chemistry/genetics/*metabolism OBPs;" |
| Notes: | "MedlineHu, Liming Chen, Binghan Liu, Kai Yu, Guohui Chen, Yirui Dai, Jianqing Zhao, Xiaojuan Zhong, Guohua Zhang, Youjun Shen, Jianmei eng 2021/01/05 J Agric Food Chem. 2021 Jan 13; 69(1):126-134. doi: 10.1021/acs.jafc.0c05945. Epub 2021 Jan 4" |
