Title: | Enhancing wine ester biosynthesis in mixed Hanseniaspora uvarum/Saccharomyces cerevisiae fermentation by nitrogen nutrient addition |
Author(s): | Hu K; Jin GJ; Xu YH; Xue SJ; Qiao SJ; Teng YX; Tao YS; |
Address: | "College of Enology, Northwest A&F University, Yangling, Shaanxi 712100, China. National Center for Yeast Technology Research and Promotion, Yichang, Hubei 443003, China; Angel Yeast Co., Ltd, Yichang, Hubei 443003, China. College of Enology, Northwest A&F University, Yangling, Shaanxi 712100, China; Shaanxi Engineering Research Center for Viti-viniculture, Yangling, Shaanxi 712100, China. Electronic address: taoyongsheng@nwsuaf.edu.cn" |
DOI: | 10.1016/j.foodres.2019.05.030 |
ISSN/ISBN: | 1873-7145 (Electronic) 0963-9969 (Linking) |
Abstract: | "The dynamic changes of wine ester production during mixed fermentation with Hanseniaspora uvarum Yun268 and Saccharomyces cerevisiae F5 was investigated at different levels and timings of nitrogen nutrient addition. Nitrogen additions were performed by supplementing yeast assimilable nitrogen (YAN) into a synthetic grape must with defined composition. Ester precursors and extracellular metabolites involved in ester synthesis were analyzed throughout the fermentation. Results showed that nitrogen additions covering 50-200?ª+mg/L YAN at the point of yeast inoculation slightly affected yeast competition and ester profiles. Interestingly, when YAN was supplemented in the mid-stage, the survival of H. uvarum Yun268 was enhanced, resulting in more than a 2-fold increase in the levels of higher alcohol acetates compared to that at the initial stage. Furthermore, carbon fluxes may be redistributed in the central pathway, which contributed to the production of medium-chain fatty acids and eventually triggered a 1.2-fold elevation in corresponding ethyl ester levels" |
Keywords: | Acetic Acid/analysis Citric Acid/analysis Esters/*analysis *Fermentation Food Microbiology Hanseniaspora/*metabolism Malates/analysis Nitrogen/*metabolism Saccharomyces cerevisiae/*metabolism Succinic Acid/analysis Vitis/chemistry Volatile Organic Compoun; |
Notes: | "MedlineHu, Kai Jin, Guo-Jie Xu, Yin-Hu Xue, Shi-Jin Qiao, Shu-Jing Teng, Yu-Xi Tao, Yong-Sheng eng Research Support, Non-U.S. Gov't Canada 2019/07/10 Food Res Int. 2019 Sep; 123:559-566. doi: 10.1016/j.foodres.2019.05.030. Epub 2019 May 21" |