| Title: | Changes of breath volatile organic compounds in healthy volunteers following segmental and inhalation endotoxin challenge |
| Author(s): | Holz O; van Vorstenbosch R; Guenther F; Schuchardt S; Trinkmann F; van Schooten FJ; Smolinska A; Hohlfeld JM; |
| Address: | "Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Clinical Airway Research, Hannover 30625, Germany. Member of the German Center for Lung Research (BREATH), Hannover, Germany. Department of Pharmacology and Toxicology, NUTRIM School of Nutrition and Translational Research, Maastricht University, Maastricht, The Netherlands. Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Bio- and Environmental Analytics, Hannover, DE 30625, Germany. Pneumology and Critical Care Medicine, Thoraxklinik at University Hospital Heidelberg, Translational Lung Research Center Heidelberg (TLRC), Heidelberg 69126, Germany. Department of Respiratory Medicine, Hannover Medical School (MHH), Hannover, Germany" |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "It is still unclear how airway inflammation affects the breath volatile organic compounds (VOCs) profile in exhaled air. We therefore analyzed breath following well-defined pulmonary endotoxin (lipopolysaccharide, LPS) challenges. Breath was collected from ten healthy non-smoking subjects at eight time points before and after segmental and whole lung LPS inhalation challenge. Four Tenax-TA(R) adsorption tubes were simultaneously loaded from an aluminum reservoir cylinder and independently analyzed by two research groups using gas chromatography-mass spectrometry. Airway inflammation was assessed in bronchoalveolar lavage (BAL) and in sputum after segmental and inhaled LPS challenge, respectively. Segmental LPS challenge significantly increased the median (interquartile range, IQR) percentage of neutrophils in BAL from 3.0 (4.2) % to 64.0 (7.3) %. The inhalation challenge increased sputum neutrophils from 33.9 (26.8) % to 78.3 (13.5) %. We observed increases in breath aldehydes at both time points after segmental and inhaled LPS challenge. These results were confirmed by an independent laboratory. The longitudinal breath analysis also revealed distinct VOC patterns related to environmental exposures, clinical procedures, and to metabolic changes after food intake. Changes in breath aldehydes suggest a relationship to LPS induced inflammation compatible with lipid peroxidation processes within the lung. Findings from our longitudinal data highlight the need for future studies to better consider the potential impact of the multiple VOCs from detergents, hygiene or lifestyle products a subject is continuously exposed to. We suspect that this very individual 'owncloud' exposure is contributing to an increased variability of breath aldehydes, which might limit a use as inflammatory markers in daily clinical practice" |
| Keywords: | Aldehydes Breath Tests/methods Endotoxins Healthy Volunteers Humans Inflammation Lipopolysaccharides *Volatile Organic Compounds/analysis airway inflammation breath analysis non-invasive monitoring oxidative stress; |
| Notes: | "MedlineHolz, Olaf van Vorstenbosch, Robert Guenther, Frank Schuchardt, Sven Trinkmann, Frederik van Schooten, Frederik-Jan Smolinska, Agnieszka Hohlfeld, Jens M eng Research Support, Non-U.S. Gov't England 2022/04/03 J Breath Res. 2022 May 3; 16(3). doi: 10.1088/1752-7163/ac6359" |
